Treatment of PC-3 cells with ultrasound combined with microbubbles induces distinct alterations in the expression of Bcl-2 and Bax  

作　　者：BAI WenKun YANG ShaoLing SHEN E ZHANG JiZhen SHEN ZhiYong HU Bing 

机构地区：[1]Shanghai Jiao Tong University Affiliated 6th People's Hospital,Shanghai Institute of Ultrasound in Medicine

出　　处：《Chinese Science Bulletin》2013年第28期3535-3540,共6页

基　　金：supported by the National Natural Science Foundation of China(81271597);the Major Infrastructure Projects of Shanghai Science and Technology(10JC1412600)

摘　　要：The objective of the present study was to investigate whether ultrasound combined with microbubbles induces apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable mechanism.We used ultrasound in continuous wave mode with a frequency of 21 kHz and a spatial-average temporal-average intensity of 46 mW/cm2.Ultrasound combined with microbubbles(200μL SonoVue)was used to treat androgen-independent human prostate cancer PC-3 cells for 30 s.PC-3 cells were divided into three groups:the control group,the ultrasound group and the ultrasound combined with microbubbles group.Immediately after treatment,trypan blue exclusion was used to assess cell viability.Cell apoptosis at 24 h after treatment was measured using transmission electron microscopy and flow cytometry.Western blotting was used to evaluate the expression of the apoptosis-related proteins,Bcl-2 and Bax.Ultrasound combined with microbubbles had a minimal effect on the viability of PC-3cells and induced minimal levels of cell lysis.The level of apoptosis in PC-3 cells induced by this modality was significantly higher than in controls(12.77±0.31%vs.2.56±0.22%,P<0.01).Treatment with ultrasound combined with microbubbles increased the expression of Bax,a pro-apoptotic protein,and decreased the expression of Bcl-2,an anti-apoptotic protein.It was concluded that ultrasound combined with microbubbles induces apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.The objective of the present study was to investigate whether ultrasound combined with microbubbles induces apoptotic cell death in androgen-independent prostate cancer cells and to identify the probable mechanism. We used ultrasound in continuous wave mode with a frequency of 21 kHz and a spatial-average temporal-average intensity of 46 mW/cm2. Ultrasound combined with microbubbles （200 μL SonoVueTM） was used to treat androgen-independent human prostate cancer PC-3 cells for 30 s. PC-3 cells were divided into three groups： the control group, the ultrasound group and the ultrasound combined with microbubbles group. Immediately after treatment, trypan blue exclusion was used to assess cell viability. Cell apoptosis at 24 h after treatment was measured using transmission electron microscopy and flow cytometry. Western blotting was used to evaluate the expression of the apoptosis-related proteins, Bcl-2 and Bax. Ultrasound combined with microbubbles had a minimal effect on the viability of PC-3 cells and induced minimal levels of cell lysis. The level of apoptosis in PC-3 cells induced by this modality was significantly higher than in controls （12.77±0.31% vs. 2.56±0.22%, P〈0.01）. Treatment with ultrasound combined with microbubbles increased the expression of Bax, a pro-apoptotic protein, and decreased the expression of Bcl-2, an anti-apoptotic protein. It was concluded that ultrasound combined with microbubbles induces apoptotic cell death in human prostate cancer PC-3 cells through down-regulation of Bcl-2 and up-regulation of Bax.

关 键 词：超声波频率 BCL-2 细胞死亡 结合治疗 BAX 微泡 诱导 WESTERN印迹法 

分 类 号：R737.25[医药卫生—肿瘤]