CDH4对涎腺腺样囊性癌细胞侵袭力的影响  

Effect of CDH4 on Invasiveness of Salivary Adenoid Cystic Carcinoma Cells

作　　者：詹杭[1] 谢健[2] 王锦锋[3] 丁林灿[2] 苏柏华[2] 卢友光[2] 

机构地区：[1]福建医科大学教学医院,莆田市第一医院口腔科,莆田351100 [2]福建医科大学附属口腔医院预防科,福州350002 [3]郑州大学第四附属医院口腔预防科,郑州450044

出　　处：《福建医科大学学报》2013年第3期137-140,共4页Journal of Fujian Medical University

基　　金：福建省科技厅科技计划重点项目(2011Y0025);福建省自然科学基金项目(2011J01167);莆田市科技局资金资助(2010s08-2)

摘　　要：目的探讨CDH4对涎腺腺样囊性癌(SACC)细胞侵袭和转移能力的影响。方法以人SACC高转移细胞株SACC-M为研究对象,利用CDH4小分子干扰RNA对CDH4基因进行沉默,Western blot检测转染前后CDH4蛋白的表达变化,体外侵袭实验比较细胞侵袭能力的变化,体外迁移运动实验比较细胞运动能力的改变。结果 CDH4siRNA明显下调CDH4基因的表达。CDH4表达下调后,SACC-M细胞体外侵袭能力显著降低,体外迁移运动能力明显降低。结论 CDH4可以明显地促进SACC细胞的体外侵袭和迁移运动,提示CDH4可能在SACC的恶性进展中起着重要作用。Objective To study the effect of CDH4 on the invasion and migration of human sali-vary adenoid cystic carcinoma cells in vitro . Methods The highly metastatic human salivary adenoid cystic carcinoma cell line SACC-M was transfected by CDH4 siRNA to silence CDH4 gene . The protein expression of CDH4 ,before and after siRNA transfection ,was examined by Western blot . At the same time ,the invasive capability and migration capability were evaluated by tumor invasion assay and migration assay ,respectively . Results CDH4 siRNA transfection specifically down-regulated the CDH4 protein in SACC-M cells . The downregulation of CDH4 suppressed the invasive capability and migration capability of SACC-M cells . Conclusion CDH4 obviously promotes the invasion and migration of SACC cells in vitro . These results suggest that CDH4 may play an important role in the malignant progression in sali-vary adenoid cystic carcinoma .

关 键 词：RNA 小分子干扰 钙黏着糖蛋白类 癌 腺样囊性 肿瘤侵润 

分 类 号：R739.8[医药卫生—肿瘤]