5-氮杂胞苷对SMMC-7721细胞增殖与侵袭的影响及作用机制  

The effect and the possible mechanism of 5-Aza-CdR on proliferation and invasiveness of human hepatocellular carcinoma cell strain SMMC-7721

作　　者：段建文[1] 吴晓[1] 叶海林[1] 符俊惠[1] 吴志伟[1] 张启瑜[1] 

机构地区：[1]温州医学院第一附属医院肝胆外科,浙江温州325000

出　　处：《肝胆胰外科杂志》2013年第5期398-401,共4页Journal of Hepatopancreatobiliary Surgery

基　　金：浙江省外科学重中之重学科资助项目(2008-255)

摘　　要：目的观察5-氮杂胞苷(5-Aza-CdR)对人肝癌细胞株SMMC-7721细胞增殖及侵袭的影响,并探讨其可能的机制。方法常规方法培养SMMC-7721细胞,加入不同浓度的5-氮杂胞苷,以CCK-8法测定终浓度为0、5、10、25、50 umol/L的5-氮杂胞苷对SMMC-7721细胞作用24、48、72 h后增殖的影响,并计算细胞生长抑制率;Transwell小室侵袭实验检测各不同浓度5-氮杂胞苷处理SMMC-7721细胞后24 h后的细胞侵袭能力特点;Western blotting法检测Caspase-3、MMP-2的表达。结果 5-氮杂胞苷能显著抑制人肝癌细胞株SMMC-7721细胞增殖,并呈时间浓度依赖性(P<0.05);与对照组相比,随5-氮杂胞苷浓度增加Caspase-3明显增加,MMP-2蛋白表达量明显增加。结论 5-氮杂胞苷对人肝癌细胞株SMMC-7721细胞增殖有明显抑制作用,且表现为时间浓度依赖性,其机制可能与上调Caspase-3蛋白表达量,诱导细胞凋亡,及对细胞的直接毒性作用有关。5-氮杂胞苷能增强人肝癌细胞株SMMC-7721细胞的侵袭能力,其机制可能与上调MMP-2蛋白表达量有关。Objective To study the effect and the possible mechanism of 5-Aza-CdR on proliferation andinvasiveness of human hepatocellular carcinoma cell strain SMMC-7721 in vitro. Methods Conventional cell culture method was used, different concentrations of 5-Aza-CdR were added, the effect of cell proliferation was measured with CCK-8 colorimetric assay after 24, 48, 72 h, and the growth inhabitation ratio was calculated; the effect of invasiveness was measured with transwell assay after 24 h. Caspase-3 and MMP-2 were detected by Western blotting. Results 5-Aza-CdR could inhibit SMMC-7721 growth in concentration-and time -dependent manners （P〈0.05）; compared with controls, Caspase-3 was increased obviously, MMP-2 was increased obvi ously too （P〈0.05）. Conclusion 5-Aza-CdR can inhibit SMMC-7721 growth and abate invasion ability, its mechanism maybe related to up-regulate the expression of Caspase-3, induce cell apoptosis and direct cell toxic effect, meanwhile up-regulate the expression of MMP-2.

关 键 词：5-氮杂胞苷 癌 肝细胞 增殖与侵袭 半胱氨酸天冬氨酸蛋白酶-3 基质金属蛋白酶-2 

分 类 号：R735.7[医药卫生—肿瘤]