实验性慢性胰腺炎大鼠骨髓间充质干细胞的检测  

Changes of reproductive activity of bone marrow mesenchymal stem cells in experimental chronic pancreatitis rats

作　　者：董育玮[1] 赵航[1] 张汝玲[1] 陆伦根[1] 王兴鹏[1] 

机构地区：[1]上海交通大学附属第一人民医院消化科,上海200080

出　　处：《诊断学理论与实践》2013年第3期290-293,共4页Journal of Diagnostics Concepts & Practice

基　　金：国家自然科学基金(81000165)

摘　　要：目的:检测实验性慢性胰腺炎(chronic pancreatitis,CP)大鼠造模后不同时间段内,骨髓间充质干细胞(mesenchymal stem cells,MSCs)的数量和增殖能力。方法:采用精氨酸腹腔注射法建立CP大鼠模型,并分别于造模后3、7、14 d获取大鼠骨髓细胞行原代培养,CFU-F计算克隆形成率。培养的细胞分别标记荧光抗体anti-CD29-PE/CY5、anti-CD44-FITC、anti-CD45-PE后,采用流式细胞仪检测MSCs细胞群CD29+CD44+CD45-占总体细胞的比例。结果:CP造模后3、7、14 d的原代培养骨髓细胞的克隆形成分别为(19.33±3.56)个、(24.67±4.89)个和(18.33±2.73)个,均显著高于对照组(4.83±1.72)个(P<0.01),获取的MSCs细胞CD29+CD44+CD45-百分比分别为(7.27±0.82)%、(7.94±2.11)%、(5.26±0.66)%,CP大鼠建模后3、7、14 d,获取MSCs比例显著高于对照组(3.37±0.55)%(P<0.05)。结论:CP大鼠建模后不同时间段MSCs数量和增殖能力发生改变,经历了先增加后减少的过程。Objective： To analyze the percentage and proliferation capacity of MSCs at different time points after CP induction. Methods： Experimental CP was induced in rats by intraperitoneal injection of L-arginine. Animals were sacrificed at 3 d, 7 d and 14 d after model induction. Bone marrow cells were implanted for primary cuhure. Colony formation rate of bone marrow ceils was calculated by CFU-F method. Surface markers CD29,CD44,CD45 on primary culture bone marrow cells were detected by flow cytometry,aud percentages of CD29＋, CD44＋CD45- cells （MSCs） in different groups were calculated. Results： Colony formation rates in 3 d,7 d and 14 d groups were 19.33±3.56,24.67± 4.89 and 18.33±2.73,respectively,significantly higher than that of control （4.83±1.72,P〈0.01）. Percentages of CD29＋, CD44, CD45-Cells （MSCs） were （7.27±0.82）%, （7.94±2.11）% and （5.26±0.66）%,respectively, significantly higher than that of control group （3.37＋0.55）% （P〈0.05）. Conclusions： The percentage and proliferation capacity of bone marrow MSCs changes during the course of CP, with a first increasing then reducing process.

关 键 词：慢性胰腺炎 骨髓间充质干细胞 大鼠 细胞增殖 

分 类 号：R576[医药卫生—消化系统]