不同麻醉药对小鼠缺氧耐受性的影响及机制研究  

Study on effect of different anaesthetics on hypoxia tolerance and possible mechanisms in mice

作　　者：刘秋晨[1] 张驰[1] 施春花[1] 韩玲[1] 杜晓秋[1] 戴骏琪[1] 谢可鸣[2] 孙晓东[2] 张克平[3] 刘立民[2] 

机构地区：[1]苏州大学基础医学与生物科学学院,江苏苏州215123 [2]苏州大学病理生理学教研室,江苏苏州215123 [3]苏州大学实验中心,江苏苏州215123

出　　处：《中国医药科学》2013年第18期29-31,共3页China Medicine And Pharmacy

基　　金：江苏省高等教育教学改革研究项目(SG315935)

摘　　要：目的研究不同麻醉药物(戊巴比妥钠、三溴乙醇、乌拉坦和水合氯醛)对小鼠缺氧耐受性的影响及相关机制。方法小鼠随机分成6组:生理盐水对照组、普萘洛尔对照组、戊巴比妥钠组、三溴乙醇组、乌拉坦组、水合氯醛组。腹腔注射给药,缺氧瓶法测定小鼠缺氧耐受时间及耗氧率,并利用生物信号采集处理系统记录心率变化。观察常温下不同药物对小鼠缺氧耐受时间的影响,并分析缺氧耐受时间与耗氧率、心率之间的关系。结果戊巴比妥钠、三溴乙醇、乌拉坦和水合氯醛均显著延长小鼠的缺氧耐受时间(P<0.01),其中三溴乙醇和水合氯醛作用明显高于其他组。戊巴比妥钠、三溴乙醇、乌拉坦和水合氯醛均降低小鼠的耗氧率(P<0.05),其中三溴乙醇和水合氯醛的降低作用最显著。三溴乙醇、水合氯醛除降低小鼠耗氧率外,还显著降低小鼠心率(P<0.05)。结论缺氧耐受性与耗氧率和心率有关。戊巴比妥钠、乌拉坦可通过降低小鼠耗氧率增强缺氧耐受性,而三溴乙醇和水合氯醛通过降低耗氧率和降低心率增强小鼠缺氧耐受性。Objective To explore the hypoxia tolerance of mice induced by different anaesthetics （pelhobarbitalum, tribromoethanol, urethane, and chloral hydrate） and the possible mechanisms. Methods Mice were randomly divided into six groups according to the material injected into their abdominal cavity： physiological saline group, propranolol group, pelltobarbitalum group, tribromoethanol group, urethane group, and chloral hydrate group. After intraperitoneal injection, hypoxia tolerance time and oxygen consumption were measured using the hypoxia bottles and the heart rate was recorded by Medlab. The effects of different anaesthetics on the hypoxia tolerance of mouse were investigated and the relationship between hypoxia tolerance time, oxygen consumption rate and heart rate were explored. Results Pelhobarbitalum, tribromoethanol, urethane, and chloral hydrate all could significantly prolonged the survival time of mice （P 〈 0.05）, especially tribromoethanol and chloral hydrate. At the same time, oxygen consumption rate of the mice was significantly decreased by the four anaesthetics （P 〈 0.05）, especially by tribromoethanol and chloral hydrate. Beside the oxygen consumption rate, tribromoethanol and chloral hydrate also significantly reduce the heart rate of mice （P 〈 0.05）. Conclusion Hypoxia tolerance has relationship with oxygen consumption and heart rate. Our results suggested that pelhobarbitalum and urethane could improve the hypoxia tolerance in mice through decreased oxygen consumption rate, whereas tribromoethanol and chloral hydrate improve the hypoxia tolerance by decreased oxygen consumption rate and heart rate.

关 键 词：麻醉药 缺氧耐受性 小鼠 

分 类 号：R364.4[医药卫生—病理学]