节杆菌YB6发酵滤液稳定性测定及其对盆栽番茄青枯病的防治效果  

Stability test of ferment filtrate by Arthrobacter YB6 strain and its control effect on Ralstonia solanacearum

作　　者：黄明媛[1] 顾文杰[1] 张发宝[1] 卢钰升[1] 解开治[1] 蒋瑞萍[1] 

机构地区：[1]广东省农科院农业资源与环境研究所/农业部南方植物营养与肥料重点实验室/广东省养分资源循环利用与耕地保育重点实验室,广东广州510640

出　　处：《广东农业科学》2013年第18期75-78,共4页Guangdong Agricultural Sciences

基　　金：广东省科技成果转化启动项目(2010B060600007);广东省农科院土壤肥料研究所科研创新平台建设(2012B060600010)

摘　　要：节杆菌YB6(Arthrobacter spp.)为番茄青枯病拮抗菌株,其发酵滤液稳定性测定结果表明,该发酵滤液对热、酸碱及蛋白酶K敏感,在60℃及其以上温度失去抑菌活性;强酸强碱条件和蛋白酶K浓度1.5 mg/mL以上均会使其活性显著下降;对光照和紫外线稳定,5 h紫外线照射和35 000 lx光照条件下抑菌活性无显著变化。抗菌谱研究显示,对测试的8种病原菌均有抑制效果,其中对甘蔗黑粉病和小麦赤霉病抑制效果较强,抑菌率分别达88.6%和80.2%。以蘸根对番茄青枯病的防效最好,其防治率达到65.0%。A rthrobacter YB6 strain is an antagonistic bacterium against Ralstonia solanacearum. In the stability test, the ferment filtrat was sensitive to heat, acid-alkali and protease K, it had no antibacterial activity when the temperature was above 60~C, the antibacterial activity would decrease significantly under the condition of strong acid-alkali or the concentration of protease K over 1.5 mg/mL. However, the ferment filtrat had strong stability against light and UV. The antibacterial actiwity was still stable under the condition of UV irradiation for 5 h or 35 000 L light. The ferment fihrat had wide antibacterial spectrum, and had inhibiting effect against 8 pathogen in the test. It had the best inhibiting effect against Ustilago scitaminea Syd. and Fusarium graminecrum, the bacteriostatic rate achieved 88.6% and 80.2%, respectively. The inhibition activity against R. solanacearum by the dipping inoeulum method was the best in pot experiments and its biocontrol rate reached 65.0%.

关 键 词：节杆菌 番茄青枯病 发酵滤液 抑菌活性 防治效果 

分 类 号：S436.412.15[农业科学—农业昆虫与害虫防治]