环氧化酶-2和β-连环蛋白及Survivin在舌癌中的表达及相关性研究  

The expressions of Cox-2,β-catenin and Survivin in tongue carcinoma and their correlation

作　　者：王鹏[1] 王敬[1] 李乐[1] 霍峰[1] 王笑茹[1] 李春辉[2] 

机构地区：[1]承德医学院附属医院口腔科,河北省承德市067000 [2]承德医学院附属医院病理科,河北省承德市067000

出　　处：《河北医药》2013年第19期2893-2895,共3页Hebei Medical Journal

基　　金：河北省科学技术研究与发展支撑计划项目(编号:09276101D-44)

摘　　要：目的研究人舌癌组织中环氧化酶-2(Cox-2)、β-连环蛋白(β-catenin)及Survivin在基因水平的表达及相互关系,为舌癌的诊断、治疗及预后提供参考依据。方法应用逆转录-聚合酶链反应(RTPCR)方法检测Cox-2 mRNA、β-catenin mRNA及Survivin mRNA在60例舌癌和30例正常口腔黏膜中的表达情况,分析它们在舌癌组织中表达的差异性和相关性。结果 Cox-2 mRNA和Survivin mRNA在舌癌组织中呈强阳性表达,而在正常口腔黏膜均为阴性表达,差异有统计学意义(P<0.01);β-catenin mRNA在舌癌和正常黏膜中均有表达,但表达量差异有统计学意义(P<0.05)。低分化舌癌中Cox-2 mRNA、β-catenin mRNA及Survivin mRNA的表达量高于高中分化舌癌中的表达量(P<0.05)。Cox-2、β-catenin及Survivin在舌癌中的表达相互呈正相关(P<0.01)。结论 Cox-2、β-catenin及Survivin在舌癌的发生发展过程中起重要作用,Cox-2、β-catenin及Survivin之间可能存在相互协同作用。Objective To investigate the expressions of cyclooxygenase-2 （ Cox-2）, β-catenin and Survivin in tongue carcinoma in order to provide important clinical evidence for early diagnosis, therapy and prognosis of tongue carcinoma. Methods The expressions of Cox-2 mRNA, 13-catenin mRNA and Survivin mRNA were detected by RT-PCR in 60 cases of primary tongue carcinoma and 30 cases of normal oral mucosa, and the difference of expressions and correlation among them were analyzed. Results The expressions Cox-2 mRNA and Survivin mRNA were strongly positive in tongue cancer, however, which were negative in normal oral mucosa （ P 〈 0.01 ） ; there was a significant difference in β-catenin expression quantity between tongue cancer and normal oral mucosa （ P 〈 0.05 ）. The expression quantity of Cox-2 mRNA, β-catenin mRNA and Survivin mRNA in poorly differentiated tongue cancer was significantly higher than that in well-differentiated and moderately-differentiated tongue cancer （ P 〈 0.05 ）. There was a positive correlation in their expressions in tongue cancer among Cox-2, β-catenin and Survivin （ P 〈 0.01 ）. Conclusion Cox-2, β-catenin and Survivin play an important role in the pathogenesis and development of tongue cancer, and there maybe a synergism among Cox-2,β-catenin and Survivin.

关 键 词：舌癌 环氧化酶-2 Β-连环蛋白 SURVIVIN 逆转录-聚合酶链反应 

分 类 号：R739.8[医药卫生—肿瘤]