小鼠脑缺血/再灌注后细胞周期相关蛋白在神经元的表达规律  

Expression of cyclin in neurons after cerebral ischemia/reperfusion in mice

作　　者：乔海兵[1] 尚君璟[2] 接近[2] 兰依娜[2] 赵亮[2] 史志远[2] 石静[2] 

机构地区：[1]山西医科大学汾阳学院解剖学教研室,汾阳032200 [2]山西医科大学汾阳学院

出　　处：《山西医科大学学报》2013年第9期671-674,749,750,共6页Journal of Shanxi Medical University

基　　金：山西省高等学校大学生创新创业训练基金资助项目(2012392)

摘　　要：目的探讨小鼠脑缺血/再灌注后细胞周期蛋白(cyclin D1)与细胞周期蛋白依赖蛋白激酶(CDK4)在神经元的表达规律。方法 78只小鼠随机分为假手术组和缺血组,采用线栓法制作小鼠局灶性脑缺血/再灌注(I/R)模型,缺血组在缺血40 min后分为再灌注3 h,5 h,12 h,1 d,3 d和5 d组。选用缺血40 min再灌注1 d的脑组织进行TTC染色,以确认模型是否成功。采用免疫双重荧光染色法观察细胞周期相关蛋白cyclin D1和CDK4在神经元中的表达。结果小鼠局灶性脑缺血后再灌注1 d,cyclin D1在神经元中的表达达高峰,与对照组和3 d、5 d组比较,差异有统计学意义(P<0.05)。CDK4在神经元中的表达缺血后再灌注3 d达高峰,与对照组和1 d、5 d组比较,差异具有统计学意义(P<0.05)。cyclin D1和CDK4主要在半暗带的神经元中表达。结论脑缺血后cyclin D1与CDK4对神经元可能起修复作用,并可抑制神经元凋亡。Objective To investigate the changes of cell cycle protein D1 （ cyclin D1 ） and cyclin-dependent kinase 4 （CDK4） in neu- rons after cerebral ischemia/reperfusion in mice. Methods Mice were randomly divided into sham group and isehemia group. The fo- cal cerebral ischemia/reperfusion（I/R） models in mice were made by sature method. After isehemia for 40 rain, ischemic mice were di- vided into reperfusion 3 h,5 h,12 h,1 d,3 d and 5 d groups. Brain tissues after 40 rain isehemia and 1 d reperfusion were stained to confirm the model successful or not. Expression of cyelin D1 and CDK4 was observed by double immunofluoreseent staining. Results Cyelin D1 expression in neurons peaked after cerebral ischemia and 1 d reperfusion,and significantly higher than those in control group and 3 d, 5 d groups （ P 〈 0.05 ）. CDK4 expression in neurons peaked after cerebral ischemia and 3 d reperfusion, and significant- ly higher than those in control group and 1 d,5 d groups （P 〈 0.05 ）. Cyclin D1 and CDK4 mainly expressed in the half dark zone of neurons. Conclusion Cyclin D1 and CDK4 could repair the neurons and decrease the apoptosis after cerebral ischemia.

关 键 词：神经元 CYCLIND1 CDK4 脑缺血 

分 类 号：R364[医药卫生—病理学]