兔脑血管痉挛前后基底动脉中PDEⅤ表达变化  

PDE Ⅴ Expression Changes in Basilar Artery before and after Cerebral Vasospasm in Subarachnoid Hemorrhage Rabbits

作　　者：黄德章[1] 李壮志[1] 张学军[1] 王志刚[2] 

机构地区：[1]山东省威海市立医院,山东威海264200 [2]山东大学第二医院

出　　处：《中国医学创新》2013年第26期1-3,共3页Medical Innovation of China

摘　　要：目的:探讨兔蛛网膜下腔出血脑血管痉挛前后基底动脉中PDEⅤ的表达变化。方法:制作脑血管痉挛动物模型,空白对照组、实验对照组与SAH组均于制模后第3天行TCD与选择性椎-基底动脉造影,检测兔脑血管痉挛的发生及变化,检测完后,取其基底动脉,制成病理切片备用。通过免疫组化检测兔基底动脉中PDEⅤ的表达。结果:各配伍组中TCD测得的基底动脉血流速度、DSA测得的基底动脉管径值及免疫组化阳性表达产物的SUM值不等或不全相等(P<0.05),经Student-Newman-Keuls检验对各配伍组数据进行两两比较,SAH组与空白对照组、SAH组与实验对照组TCD测得的基底动脉血流速度、DSA测得的基底动脉管径值及免疫组化阳性表达产物的SUM值不等(P<0.05),空白对照组与实验对照组TCD测得的基底动脉血流速度、DSA测得的基底动脉管径值及免疫组化阳性表达产物的SUM值差异无统计学意义(P>0.05)。结论:兔蛛网膜下腔出血脑血管痉挛后PDEⅤ在其基底动脉中明显表达,推测PDEⅤ表达可能与脑血管痉挛发生有关。Objective：To study the basilar artery expression changes of PDE Ⅴ before and after cerebral vasospasm in subarachnoid hemorrhage rabbits.Method：Made the vasospasm rabbits model.In the all groups,the DSA and TCD were made to assess the cerebral vasospasm on the 3rd day.After that,sacrifice the animal models,and then take the basal arteries as the specimen.The specimen were made paraffin-embedded sections for Immunohistochemical examination.Result：The velocity of basal arteries by TCD ﹑ the arteries diameters by DSA and the SUM by IHC in all groups were not equal or not completely equa（P〈0.05）.And Significantstatistics differences were found in multiple comparison（P〈0.05）,between SAH and blank contrast group,SAH and experimental contrast group by Student-Newman-Keuls test.But no differences were found between blank and experimental contrast group（P〈0.05）.Conclusion：The PDE Ⅴ express obviously in basilar artery of subarachnoid hemorrhage rabbits.So the PDE Ⅴ and the CVS may be closelyy related.

关 键 词：蛛网膜下腔出血 脑血管痉挛 磷酸二酯酶Ⅴ 兔 

分 类 号：R743.3[医药卫生—神经病学与精神病学]