大体积进样-逆电渗流堆积-毛细管区带电泳分离测定厚朴酚、绿原酸及咖啡酸  

Determination of Magnolol,Chlorogenic Acid,Caffeic Acid in Qinggan Lidan Oral by Capillary Electrophoresis with LVSS-Reverse Current Flow Accumulation-CZE

作　　者：张瑞瑞[1] 李利军[1] 杨兰兰[1] 孙科[1] 崔越[1] 崔福海[1] 罗应[1] 李彦青[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006

出　　处：《分析测试学报》2013年第9期1106-1111,共6页Journal of Instrumental Analysis

基　　金：国家自然科学基金项目(31060219)

摘　　要：运用大体积进样-逆电渗流堆积-毛细管区带电泳分离测定了厚朴酚、绿原酸和咖啡酸。采用未涂层熔融石英毛细管(50 cm×50μm i.d.,有效柱长36 cm)分离;紫外检测波长为220 nm,运行缓冲液为40mmol/L四硼酸钠-20 mmol/L磷酸氢二钠(pH 9.0),分离电压16 kV,电动进样电压-12 kV,进样时间356s时达到最佳的分离效果。在优化条件下,上述3种化合物均在20 min内出峰,峰面积的RSD均小于4%。检出限分别为184.2、36.07、77.99 ng/L。将该法用于清肝利胆口服液中厚朴酚、绿原酸和咖啡酸的分离测定,结果满意。A large volume sample injection（LVSS）-reverse current flow accumulation capillary zone electrophoresis（CZE） method was used for the simultaneous separation and determination of magnolol,chlorogenic acid and caffeic acid in Qinggan Lidan oral.The result indicated that a best separation of three materials were obtained on an uncoated fused silica capillary column（50 cm×50 μm,effective length 36 cm） by using background electrolyte containing 40 mmol/L Na2B4O7,20mmol/L NaH2PO4,pH 9.0）.The detection wavelength was set at 220 nm,with the separation voltage at 16 kV,the sample injection voltage at-12 kV and the injection time in 356 s.Under the optimum conditions, the separation of three compounds was performed within 20 min with relative standard deviations（RSDs） of peak area less than 4%.The detection limits（S/N=3） of magnolol,chlorogenic acid and caffeic acid were 184.2,36.07,77.99 ng/L,respectively.

关 键 词：大体积进样 区带电泳 厚朴酚 绿原酸 咖啡酸 

分 类 号：O657.3[理学—分析化学] O614[理学—化学]