NGF对LASIK及Epi-LASIK术后角膜中TrkA受体表达作用的研究  

Effect of nerve growth factor on expression of TrkA in cornea after LASIK or Epi-LASIK

作　　者：余洁[1] 赵海岚[1] 吴苗琴[1] 

机构地区：[1]浙江省人民医院眼科中心,杭州310014

出　　处：《浙江医学》2013年第16期1478-1480,共3页Zhejiang Medical Journal

基　　金：浙江省医药卫生科技计划项目(2006A07)

摘　　要：目的观察兔眼准分子激光原位角膜磨镶术(LASIK)及准分子激光上皮瓣下角膜磨镶术(Epi-LASIK)术后局部点用神经生长因子(NGF)后角膜组织中酪氨酸激酶受体A(TrkA)的表达情况,探索术后干眼症的防治方法。方法将30只新西兰纯种兔,抽取15只30眼接受LASIK手术,余接受Epi-LASIK手术。术后每只兔子一眼滴NGF滴眼液,为实验眼,对侧眼滴0.9%氯化钠溶液,为对照眼。1周后进行免疫组化分析,检测角膜上皮细胞层以及角膜基质层中TrkA的表达情况。结果观察TrKA的表达,接受LASIK或Epi-LASIK手术后,滴用NGF滴眼液的眼均较对照眼强。LASIK组以及Epi-LASIK组滴用NGF眼两者比较TrkA的表达无统计学差异。结论滴用NGF的兔眼,其LASIK或Epi-LASIK术后TrkA受体表达均较高,可能有助于防治近视激光术后干眼症。Objective To investigate the effect of nerve growth factor （NGF） on the expression of TrkA in cornea after LASIK or Epi- LASIK. Methods Sixty eyes of 30 New Zealand white rabbits underwent LASIK or Epi- LASIK procedures with 30 eyes in each group. One eye, randomly selected in each rabbit was treated with topical NGF for 1 week, while the contralateral eye was treated with normal saline as control. The expression of NGF receptor （TrkA） on cornea was detected by histoimmuno-chemistry. Results The expression of TrkA on the cornea treated with the topical NGF after LASIK or Epi- LASIK was stronger than that of control group. There was no difference in expression of TrkA between LASIK and Epi- LASIK groups. Conclusion Topical treatment of NGF can up- regulate the expression of TrkA in the cornea of rabbits after LASIK or Epi- LASIK.

关 键 词：神经生长因子 酪氨酸激酶受体A 准分子激光原位角膜磨镶术 准分子激光上皮瓣下角膜磨镶术 干眼症 

分 类 号：R779.63[医药卫生—眼科]