乙酰胆碱在大鼠海马CA1区痛觉调制中的作用  

Influences of acetylcholine on nociceptive modulation in hippocampal CA1 area

作　　者：肖宇[1] 贾伟伟[2] 李雪[1] 卢长柱[1] 王月飞[1] 霍红[3,4] 徐满英[5] 

机构地区：[1]齐齐哈尔医学院生理学教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院机能学教研室,黑龙江齐齐哈尔161006 [3]齐齐哈尔医学院 [4]黑龙江省医院循环内科,黑龙江哈尔滨150036 [5]哈尔滨医科大学生理学教研室,黑龙江哈尔滨150081

出　　处：《中国药理学通报》2013年第9期1239-1243,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 30240058)

摘　　要：目的研究海马CA1区及其内分布的乙酰胆碱(ACh)受体在大鼠痛觉调制中的作用。方法以电脉冲刺激坐骨神经作为伤害性电刺激,用玻璃微电极细胞外记录痛反应神经元的电活动。结果侧脑室微量注射ACh能使大鼠海马CA1区痛兴奋神经元(PEN)痛诱发放电频率降低、潜伏期延长,痛抑制神经元(PIN)痛诱发放电频率增加、完全抑制时程缩短;注入ACh的M受体拮抗剂阿托品能够阻断ACh的上述效应;注入M受体激动剂毛果芸香碱产生与ACh相似的效应。结论上述结果提示,ACh通过影响海马CA1区PEN和PIN的电活动,参与了海马CA1区的痛觉调制,其机制可能是通过M受体介导而实现的。Aim To study the role of acetylcholine （ACh） and cholinergic receptor in the nociceptive modulation in hippocampal CA1 area of normal rats. Methods The trains of electric impulses applied to sciatic nerve were set as noxious stimulation. The dis- charges of pain-related neuron in the CA1 area were re- corded extracellularly by glass microcleetrode. Results The intracerebroventricular （iev） micro-injection of ACh caused the decrease in the pain-evoked discharge frequency and the prolongation of latency of pain-exci- tation neurons （PEN） , while it increased the pain-e- voked discharge frequency and shortened the inhibitory duration of pain-inhibition neurons （PIN） in hippocampal CA1 area. The ACh-induced response was significantly blocked by the M-receptor antagonist atro- pine. The effects of M-receptor agonist pilocarpine were similar to the effect of ACh. Conclusion On the basis of the above-mentioned findings, we can deduce that ACh and acetylcholine M-receptors （mAChRs） in the hippocampal CA1 area are involved in the modula- tion of nociceptive response by regulating the electric activities of PEN and PIN.

关 键 词：海马CA1区 乙酰胆碱 阿托品 毛果芸香碱 痛兴奋神经元 痛抑制神经元 

分 类 号：R-332[医药卫生] R322.81