尿皮质素诱导心肌细胞内钙离子升高与兰尼碱受体活化的关系研究  

Relationship between urocortin enhanced [Ca^(2+)]_i of neonatal rat cardiomyocytes and ryanodine receptor activation

作　　者：梁春光[1] 黄雷 戴红良[1] 刘涛[1] 

机构地区：[1]辽宁医学院分子生物学与新药开发重点实验室,辽宁锦州121000 [2]锦州市食品药品检验所,辽宁锦州121001

出　　处：《中国药理学通报》2013年第10期1401-1403,共3页Chinese Pharmacological Bulletin

基　　金：辽宁省高等学校优秀人才支持计划项目(No 2008RC33)

摘　　要：目的探讨尿皮质素(urocortin)诱导乳大鼠心肌细胞内钙离子浓度[Ca2+]i升高与兰尼碱受体(RyR)之间的关系。方法采用体外培养的乳大鼠心肌细胞进行指标测定。采用Till阳离子测定系统,以Fura-2/AM为荧光探针,观察心肌细胞[Ca2+]i瞬间变化。结果 urocortin使心肌细胞内钙离子浓度升高;兰尼碱受体抑制剂Ryanodine和IP3受体抑制剂Xestospongin C能够部分抑制urocortin诱导的心肌细胞内钙离子浓度升高,差别有统计学意义(P<0.01)。结论urocortin能够诱导心肌细胞内钙离子浓度[Ca2+]i升高,其作用可能与活化RyR和IP3受体使心肌细胞肌质网内钙离子释放有关。Aim To study the relationship between in- creased intracellular Ca2＋ （ Ca^2＋ ]i） of neonatal rat eardiomyocytes induced by urocortin （UCN） and ryano- dine receptor. Methods Cultured neonatal rat cardi- ac myoeytes were treated with UCN, and indexes were measured afterwards. [ Ca^2＋]i transient was measured by Till image system by loading of Fura-2/AM. Re- suits UCN could increase [ Ca^2＋]i, which was reversed by pretreatment of ryanodine and Xestospongin C significantly （P 〈 0. 01 ）. Conclusion UCN could enhance [ Ca^2＋]i of cultured cardiomyocytes via acti- vation of ryanodine receptor or IP3 receptor.

关 键 词：尿皮质素 细胞内钙离子 兰尼碱受体 心肌细胞 兰 尼碱 细胞培养 

分 类 号：R-332[医药卫生] R322.11