类叶升麻苷对D-半乳糖致衰老小鼠抗氧化作用的研究  

Antioxidant effects of acteoside on aged mice induced by D-galactose

作　　者：高莉[1] 林娟 张富春[1] 霍仕霞 彭晓明 闫明 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046 [2]新疆维吾尔自治区维吾尔医药研究所新疆维吾尔医方剂学重点实验室,新疆乌鲁木齐830049

出　　处：《中国药理学通报》2013年第10期1440-1443,共4页Chinese Pharmacological Bulletin

基　　金：新疆维吾尔自治区科研机构创新发展专项资金(No2012015);新疆维吾尔自治区公益性科研院所基金(NoKY2012114)

摘　　要：目的研究类叶升麻苷对D-半乳糖所致衰老小鼠的学习记忆能力和氧化作用的影响。方法将昆明种小鼠随机分为空白对照组、模型组、石杉碱甲组和类叶升麻苷高、中、低剂量组。空白对照组颈背部注射生理盐水,其余各组颈背部注射D-半乳糖(150 mg·kg-1·d-1)造模,连续90d。从第71天开始石杉碱甲组和类叶升麻苷低、中、高剂量组分别灌胃给药,连续20 d。采用Morris水迷宫测试小鼠的学习记忆能力,然后检测血清和脑组织内的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量。结果与模型组相比,给予类叶升麻苷和石杉碱甲后可以改善小鼠的学习记忆能力,其潜伏期和游泳距离均明显缩短(P<0.05)。与空白对照组相比,模型组小鼠血清和脑组织内SOD、GSH-Px活性明显降低(P<0.01),MDA含量明显升高(P<0.01)。与模型组相比,类叶升麻苷组在给药后均能明显提高小鼠血清和脑组织内SOD和GSH-Px活性(P<0.01),降低血清和脑组织内MDA含量(P<0.01)。小鼠学习记忆能力与血清和脑组织内SOD、GSHPx活性以及MDA含量相关(P<0.01)。结论类叶升麻苷能够改善小鼠学习记忆功能,提高血清和脑组织内抗氧化酶活性,降低MDA含量。Aim To study the effects of learning and memory and antioxidant effects of acteoside on aged mice induced by D-galactose （D-gal）. Methods 72 Kunming mice were randomly divided into control group, aging model group, Huperzine-A group, and acteoside high-, middle-and low-doses groups. The con- trol group mice were subcutaneously injected with nor- mal saline in the nape of the neck for 90d. The mice in the rest of the groups were subcutaneously injected with D-gal （150 mg·kg^-1·d^-1） in the nape of neck. The mice in Huperzine-A group and acteoside groups were simukaneously treated by intragastric gavage from 71d to 90d. Their learning and memory abilities were detected with a Morris test. Then the level of SOD, glutathione peroxidase （GSH-Px） and malondialdehyde （MDA） in serum and brain tissue of the mice were de- tected in each group. Results The acteoside im- proved the learning and memory abilities of the aging model group. Compared with control group, SOD, GSH-Px activity revealed a significant decrease and MAD content exhibited a significant increase in serum and brain tissue in aging model mice. Compared with aging model group, SOD and GSH-Px activity in serum and brain tissue exhibited a significant increase and MDA content decreased in acteoside treatment group. The antioxidant effects of Huperzine-A group resembled those of acteoside treatment group. Correlation analysis showed that learning and memory abilities were closely related to SOD and GSH-Px activity, and MDA content in mice serum and brain. Conclusion Acteoside im- proves the disability of learning and memeory induced by D-gal, and may have effect on suppressing the aging process through enhancing antioxidant activity, and de- creasing MDA content.

关 键 词：类叶升麻苷 学习记忆 MORRIS水迷宫 超氧化物歧化酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R-332[医药卫生] R284.1