尿激酶型纤溶系统在乳腺癌细胞侵袭中的作用  

Role of Urokinase Plasminogen Activator System in the Invasiveness of Human Breast Cancer

作　　者：徐韶华[1] 廖劲晖[1] 于伟英[1] 朱运松[1] 

机构地区：[1]上海医科大学基础医学院分子遗传学研究室,上海200032

出　　处：《上海医科大学学报》2000年第6期453-456,共4页Journal of Fudan University(Medical Science)

基　　金：国家自然科学基金 !(396 70 819)资助课题

摘　　要：目的　研究尿激酶型纤溶系统组分uPA、uPAR、tPA及PAI 1在人乳腺癌细胞侵袭中的作用。方法　以 3株具有不同侵袭转移能力的乳腺癌细胞株作为研究对象 ,应用RT PCR方法比较纤溶组分在此 3株细胞中的表达 ,牛奶板法检测细胞培养上清中的纤溶活性 ,用Boyden小室模型测定细胞侵袭能力。结果　发现MDA MB 2 31细胞表达较高水平的uPA、uPAR、PAI 1和中等水平的tPA ,无血清培养上清中总纤溶活性和uPA纤溶活性最高 ;MDA MB 435细胞表达较低水平的uPA和较高水平的tPA ,但未测出uPAR和PAI 1的表达 ,无血清培养上清中总纤溶活性较高 ,主要是tPA活性 ;MCF 7细胞表达较低水平的uPAR和较高水平的PAI 1,但未测出uPA和tPA的表达 ,无血清培养上清中几乎没有纤溶活性。与纤溶活性相一致的是 ,Boyden小室模型实验结果发现MDA MB 2 31在 3株细胞中体外侵袭能力最强 ,MDA MB 435次之 ,MCF 7则几乎无体外侵袭能力。经抗uPA和抗uPAR抗体预处理MDA MB 2 31细胞 ,分别使侵袭能力下降 83 .1%和 43.9% (P <0 .0 5 )。Purpose To investigate the role of urokinase plasminogen activator (uPA),uPA receptor (uPAR),tissue type plasminogen activator (tPA) and plasminogen activator inhibitor 1 (PAI?1) in the invasiveness of human breast cancer cells. Methods Three human breast cancer cell lines with different invasive ability were taken as research targets.RT?PCR and milk plates methods were used to detect the expression of uPA system members and the PA activities,respectively.Modified Boyden's chamber model was employed to detect the invasive ability of cancer cell. Results MDA?MB?231 could express high level of uPA,uPAR,PAI?1 and low level of tPA.MDA?MB?435 could express lower level of uPA and hight level of tPA,but no PAI?1 and uPAR were detected.MCF?7 could express lower level of uPAR and high level of PAI? 1,but no uPA and tPA were detected.MDA?MB?231 cells showed the highest total PA and uPA activity.MDA?MB?435 cells also showed high total PA activity,but almost all the activity owed to tPA.MCF?7 showed almost no PA activity.Correlated with their PA activities,MDA?MB?231 was found the most invasive in vitro ,followed by MDA?MB?435,and MCF?7 almost had no invasive ability.The antibodies against uPA and uPAR were significantly effective in reducing the matrigel invasiveness of MDA?MB?231 by approximately 83.1% and 43.9% respectively ( P <0.05). Conclusions Co expression of uPA,uPAR and PAI?1 in human breast cancer highly correlates with the invasiveness in vitro .

关 键 词：乳腺癌 肿瘤侵袭 尿激酶型纤溶系统 

分 类 号：R737.902[医药卫生—肿瘤] R73－37[医药卫生—临床医学]