色氨酸对慢性应激小鼠行为的影响  

Effect of Tryptophan on Behavior of Chronically Stressed Mice

作　　者：李萌萌[1] 崔晓[1] 朱凌峰[1] 刘航[1] 彭彦[1] 辛琳[1] 代卉[1] 朱建津[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2013年第8期838-843,共6页Journal of Food Science and Biotechnology

基　　金：国家"十二五"科技支撑计划项目(2012BAD33B00);江南大学2011年大学生创新训练计划项目(1025210232113480)

摘　　要：研究色氨酸对慢性应激小鼠行为的影响及与下丘脑5-HT的关系。将60只小鼠随机分为空白组(NC)、慢性应激模型组(CUS)、慢性应激模型+高剂量色氨酸(CUS-H)、慢性应激模型+低色氨酸(CUS-L),对CUS、CUS-H、CUS-L建立慢性应激模型,同时分别对CUS-H、CUS-L组灌胃高、低剂量的色氨酸,建模第5周开始进行旷场实验、悬尾实验、糖水消耗实验、强迫游泳实验及水迷宫实验等行为学实验,测定行为学指标,并采用ELISA法检测下丘脑5-HT含量。CUS-H组旷场实验得分显著高于CUS组与NC组(p<0.05),悬尾实验静止时间与CUS与NC组相比极显著地缩短(p<0.01),CUS-H组糖水偏好程度极显著低于CUS组(p<0.01),其强迫游泳静止时间极显著低于NC组(p<0.01);灌胃色氨酸对应激动物的学习和记忆能力有一定增强作用,但作用不显著;灌胃色氨酸可极显著地提高下丘脑5-HT含量(p<0.01)。研究结果表明,灌胃色氨酸能促进下丘脑5-HT的合成并有效增强动物的抗应激能力。抗应激能力的增强可能与下丘脑5-HT含量的提高有关。Experiments were conducted to evaluate the effect of tryptophan on behavioral reactivity of stressed mice and its relation with hypothalamic 5-hydroxy tryptamine （5-HT）.60 mice were randomly divided into 4 groups：NC group （saline administration）,CUS group （saline administration and chronic stress stimulation）,CUS-H group （high-dose tryptophan administration and chronic stress stimulation） and CUS-L group （low-dose tryptophan administration and chronic stress stimulation）.A chronic stress model was established to CUS,CUS-H and CUS-L group.Meanwhile,different levels of tryptophan were administrated to CUS-H and CUS-L group.From the fifth week of establishing the model,open-field test,tail-suspension test,sugar preference test,forced swimming test and water maze were measured,and the hypothalamic serotonin level was assayed.The results are as follows：Compared to CUS and NC group,a notable increase （p〈0.05） in open-field test score and a marked decrease （p 〈0.01）in immobile time of tail suspension test of CUS-H group were discovered,the sugar preference of CUS-H group were also decreased compared to CUS group（p〈O.01）.Besides,the immobile time of force swimming test was lower than NC group （p 〈0.01）;Learning and memorizing ability of stressed mice was indistinctively improved after tryptophan administration;The Hypothalamic 5-HT content was obviously increased （p〈0.01） after tryptophan administration.The results suggested that administration of tryptophan increase the content of hypothalamic 5-HT and effectively reverse the variation of stress resistant capability of stressed mice.The strengthened stress resistant capability might be related to the enhanced level of hypothalamic 5-HT.

关 键 词：色氨酸 慢性应激 5-羟色胺 动物行为 学习记忆能力 

分 类 号：R151[医药卫生—营养与食品卫生学]