PES1在卵巢癌中的表达及其对内皮生长因子的影响  

Expression of VEGF induced by PES1 via enhancing hypoxia inducible factor-1αexpression in epithelial ovarian cancer cells

作　　者：江絮萍[1] 叶泓[1] 

机构地区：[1]武警福建总队医院妇产科,福州350003

出　　处：《重庆医学》2013年第27期3211-3213,共3页Chongqing medicine

摘　　要：目的探讨PES1在卵巢癌中的表达及其与血管内皮生长因子(VEGF)表达的关系。方法用蛋白印迹法检测PES1在卵巢癌组织以及相应的癌旁组织中的表达。结果肿瘤组织中PES1的表达水平明显高于相应的癌旁组织。转染FLAG-PES1的CAOV-3和ES-2细胞培养液的VEGF分泌量分别由(178.0±11.8)pg/mL和(309.5±18.5)pg/mL上升到(375.0±18.3)pg/mL和(633.2±25.7)pg/mL,VEGF分泌量明显升高(P<0.01);而且VEGF相对mRNA水平也分别上升了约1.8倍和2倍(P<0.01);蛋白印迹法结果表明,PES1过表达能升高这2种细胞中缺氧诱导因子-1α(HIF-1α)的表达。荧光素酶报告基因的转录激活活性检测表明,PES1对调节VEGF的转录没有直接影响。将HIF-1αSiRNA与FLAG-PES1共转染CAOV-3和ES-2细胞后,蛋白印迹法结果表明,HIF-1αSiRNA能明显抑制PES1引起的HIF-1α升高,同时VEGF的分泌量和mRNA水平也被明显抑制。结论 PES1在卵巢癌组织中表达明显升高。Objective To investigate the expression of PES1 in ovarian cancer and its relationship with the vascular endothelial growth factor（VEGF） expression .Methods The expression of PES1 in ovarian cancer tissues and corresponding pericancerous tis-sues was detected with Western blot .Results The expression of PES1 in ovarian cancer tissues was obviously higher than that in the pericancerous tissues .The secretion amounts of VEGF in cell culture fluid of CAOV-3 and ES-2 with transfection of FLAG-PES1 were elevated from （178 .0 ± 11 .8） pg/mL and （309 .5 ± 18 .5） pg/mL to （375 .0 ± 18 .3） pg/mL and （633 .2 ± 25 .7）pg/mL respectively （P〈0 .01） ,the secretion amounts of VEGF were also increased（P〈0 .01） .The relative VEGF mRNA levels were also raised by 1 .8 times and 2 times respectively （P〈 0 .01）;Western blot simultaneously showed that the over expression of PES1 could increase the expression of HIF-1αin these two kinds of cells .The luciferase report gene transcriptional activation activity de-tection reveald that PES1 had no direct effect on the VEGF transcription .After cotransfecting HIF-1αSiRNA and FLAG-PES1 to CAOV-3 and ES-2 cells ,Western blot demonstrated that HIF-1αSiRNA could obviously inhibit the increase of HIF-1αexpression induced by PES1 ,at the same time the secretion amounts of VEGF and mRNA level were also supressed .Conclusion The expres-sion of PES1 is obviously up-regulated in ovarian cancer tissues .

关 键 词：卵巢肿瘤 血管内皮生长因子A 缺氧诱导因子1α亚基 PES1 

分 类 号：R737.31[医药卫生—肿瘤]