模拟克隆病的肉芽肿性肠炎动物模型的建立  

Establishment of a Small Animal Model of Granulomatous EnteritisMimic Crohn's Disease

作　　者：缪应雷[1] 杜艳[2] 欧阳钦[3] 

机构地区：[1]昆明医学院第一附属医院消化内科,昆明650032 [2]昆明医学院第一附属医院检验科,昆明650032 [3]华西医科大学附属一医院消化内科,成都610041

出　　处：《昆明医学院学报》2000年第4期17-20,共4页Journal of Kunming Medical College

基　　金：云南省教委青年研究基金资助!(994 2 0 36 )

摘　　要：40只地鼠随机分为两组 ,实验组 :2 0只腹腔接种副结核杆菌 ;对照组 :2 0只分别接种生理盐水和死菌 (各 10只 ) ,8个月后处死动物 ,作病理学观察 .结果 :实验组 80 % (16 /2 0 )的地鼠出现肉芽肿性肠炎 ,而对照组未见肉芽肿性肠炎和溃疡形成 .两组肉芽肿性肠炎的发病率有显著性差异 (P <0 0 5) .结果表明 :利用副结核杆菌接种地鼠 ,建立了一个模拟克隆病的肉芽肿性肠炎小动物模型 .The aetiology of Crohns disease (CD) remains unknown. Inoculumconsisted of 10?mg freshly harvested MP strain P18 in 1 0?mL Saline. At 10 weeks of age, 40 hamsters were inoculated intraperitoneally, twenty received inoculum, tenreceived dead inoculum and ten received saline. All hamsters were observed dailyfor evidence of clinical abnormality with respect to fecal consistency and weighedweekly until 8 months postinoculation. When the experiment was terminated, tissuewas obtained from intestine and evaluated macroscopically and histologically.Result showed that: Four test hamsters were found dead during weeks 2,4,8 and 20 postinfection. No control hamster died of postinoculation. The average weights of control and test hamsters prior to inoculation were similar. A statistically significant difference in the mean weights between the two groups was determined 12 weeks postinoculation and continued throughout the experimental period. Microscopically, 16 of 20 hamsters received MP showed granulomatous changes andulcerat ive format ion. But the control hamsters didnt show granulomatous enteritis.An animal model of granulomatous enteritis mimic Crohns disease has beenestablished by inoculated intraperitoneally MP.

关 键 词：副结核杆菌 肉芽肿性肠炎 动物模型 克隆病 

分 类 号：R574.62[医药卫生—消化系统]