水稻矮秆基因d-ss的遗传分析与克隆  

Genetic Analysis and Cloning of the Rice Dwarf Mutant d-ss

作　　者：罗茂春[1,2] 赵政[1] 夏令[1] 郭迟鸣[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院,厦门市植物遗传重点实验室,福建厦门361102 [2]龙岩学院生命科学学院,福建龙岩364012

出　　处：《厦门大学学报（自然科学版）》2013年第5期684-689,共6页Journal of Xiamen University：Natural Science

基　　金：国家重点基础研究发展计划(973)项目(2012CB126312)

摘　　要：通过γ射线诱变,从粳稻品种9522的M2代中筛选出一株矮秆水稻(Oryza sativa L.)突变体,定名d-ss.d-ss突变体表现为叶色深绿、短宽的叶片、以及小而圆的籽粒.以d-ss突变体与籼稻品种龙特普杂交的F2代群体为基因定位群体,利用InDel分子标记将d-ss突变位点定位在5号染色体上的InDel标记ZZ5-6和ZZ1343之间,物理距离为412kb.最终通过图位克隆的方法获得了此基因,测序结果表明此基因在编码区发生了两处缺失突变.Rice height character is one of the most important agronomic traits of rice（Oryza sativa L. ）. Generally,the clwarl vane- ties with proper plant height have a greater harvest and higher lodging resistance. Identifying new useful dwarf mutant and under- standing its regulating mechanism is an important subject for practice rice breeding. In this study, the d-ss, a mutant of Oryza satfva L. spp. japonica cv. 9522, was mutagenized by irradiation with 60 Co ＂/-ray. The d-ss mutant plants display short stem, dark green leav- es, compact panicles, and short, round grains. To map the d-ss locus, an F2 population generated by crossing between d-ss （japonica） mutant and Longtepu （indica） was analyzed. The d-ss locus was mapped to rice chromosome 5 ,between the two InDel markers,ZZS- 6 and ZZ1343. The region was delimited to about 412 kb. At last,the d-ss gene was cloned by map-based cloning. The analysis of se- quencing indicated two deletions happened in translated regions of d-ss gene.

关 键 词：水稻 矮秆 基因定位 图位克隆 

分 类 号：Q943[生物学—植物学]