慢性牙周炎患者龈沟液EA、COL-Ⅱ和KGF-1水平的变化及其意义  

Clinical Significance of Determination the Changes on Gingival Crevicular Fluid EA,COL-Ⅱand KGF-1 Levels in Patients with Chronic Periodontitis

作　　者：周敏[1,2] 

机构地区：[1]扬州大学第五临床医学院 [2]常熟市第二人民医院口腔科,215500

出　　处：《放射免疫学杂志》2013年第5期645-647,共3页Journal of Radioimmanology

摘　　要：目的:探讨慢性牙周炎(chronic periodontitis,CP)患者龈沟液(gingival crevicular fluid,GCF)弹性蛋白酶(elastase,EA)、Ⅱ型胶原酶(collagenase-Ⅱ,COL-Ⅱ)和角质细胞生长因子-1(keratinocyte growth factor-1,KGF-1)水平的变化及其临床意义。方法:正常对照组为58名正常成人和儿童。将62例CP患者根据不同病情分为:轻度(30例),中度(18例),重度(14例)三组。GCF中EA、COL-Ⅱ和KGF-1测定分别采用底物催化法和酶联免疫分析法。结果:EA水平轻度组与正常对照组比较略高,但无统计学差异(P>0.05),中度和重度组则显著高于正常对照组(P<0.05,P<0.01),重度组又分别显著高于轻度和中度组(P<0.05,P<0.01)。COL-Ⅱ水平的变化规律同EA。KGF-1水平则轻度组与正常对照组比较呈显著高水平(P<0.05),中度和重度组水平与正常对照组比较升高更为显著(P均<0.01),重度组则显著高于轻度和中度组(P<0.05,P<0.01)。结论:CP患者GCF中三项指标水平变化与病情的进展关系密切。其测定对于CP的诊断和炎症程度的评估有一定的帮助。Objective To explore the changes of gingival creviculer fluid EA, COL- Ⅱ and KGF-1 levels in patients with chronic periedontitis and the clinical significance of determination. Methods According, to the different degree of pathosis with 62 patients were divided to minor group（ n = 30）, mederater group（ n = 18 ） and severe group（ n = 14）. Their gingival crevicurlar fluid levels of COL- Ⅱ and KGF-1 （ with enzyme ;mmunoassay） , EA activity （ with substrate catalytic method） were determined in 62 patients with chronic periodoutitis and 58 healthy people served as normal control group. Results In minor group：EA level expressed slightly high- er than that of controls, but without significant difference （ P 〉 0. 05 ）, and in the moderate group and severe group： EA levels were markedly higher than those in contmls（P 〈0.05, P 〈0.01 ） ,in the severe group EA level was obviously higher than that of minor and moderate groups （P 〈0.05, P 〈0.01 ）. The gingival crevicurlar fluid levels of COL- Ⅱ in 3 groups with EA statistical results were con- sistent. The level of KGF-1 in the minor group was significantly higher than that of controls（P 〈0.05 ） ,the gingival crevicurlar fluid level of KGF-1 were remarkably higher in the mederate group and severe group compared with the control（ all P 〈0.01 ）, and the gin- givai crevicurler fluid the index level showed all apparently higher in patients of severe group than the minor and moderate groups （P 〈 0.05, P 〈0.01 ）. Corldusion 3 indexes of gingival crevicular fluid levels chanss closely related to the progress of pathosis. The de-terminations might be helpful to measure the diagnosis of CP and estimate the degree of inflammation.

关 键 词：慢性牙周炎 龈沟液 弹性蛋白酶 胶原酶-Ⅱ 角质细胞生长因子 

分 类 号：R783.5[医药卫生—口腔医学]