硅藻土/活性炭对石油降解菌群的固定化研究  

Research on Immobilization of Petroleumdegrading Bacterial Flora with Diatomite/Activated Carbon

作　　者：单海霞[1] 刘晓宇 张颖[3] 王中华[1] 何焕杰[1] 马金[1] 郭民乐[1] 

机构地区：[1]中国石化中原石油工程有限公司钻井工程技术研究院 [2]中原油田技术监测中心化工产品监测总站 [3]中原油田分公司天然气处理厂

出　　处：《油气田环境保护》2013年第4期19-21,78,共3页Environmental Protection of Oil & Gas Fields

基　　金：中国石化集团重点科技重点科技攻关项目;编号P120006

摘　　要：研究了一种石油降解菌群的固定化方法,其中菌群包括假单胞菌、芽孢杆菌和微球菌。以硅藻土/活性炭作为降解菌群的固定化载体,对最佳固定化条件进行研究,结果表明:降解菌群的最佳固定化时间16h,温度37℃,硅藻土/活性炭加入量0.1g/mL,pH值7.5,120r/min振荡16h,降解菌群固定化率达97.1%。固定化菌群用于油基钻屑中油降解,降解14d,可使钻屑中TPH含量由30 000mg/kg降至10 450mg/kg,平均油去除率达65%。A immobilization method for petroleum-degrading bacterial flora including pseudomonas,bacillus,and micrococcus is studied. This article takes diatomite/activated carbon as immobilization carrier for petroleum-degrading bacterial flora,and conducts investigation into its optimal immobilization conditions.The result shows that the optimal immobilization time for degrading bacterial flora is16hours,with the temperature of 37℃,the amount of added diatomite/activated carbon of 10g/mL,pH value of 7.5,and the shaking rate of 120r/min,under which conditions the immobilization rate achieving 97%. Oil-base drilling cuttings degrading for 14d with immobilized bacterial flora reduces TPH content in the cuttings from 30 000mg/kg to 10 450mg/kg,with average oil removal rate up to 65%.

关 键 词：石油降解菌 固定化 硅藻土 活性炭 

分 类 号：X172[环境科学与工程—环境科学]