光叶紫花苕种子贮藏蛋白分析  

Analysis on seed storage proteins of Vicia villosa var. glabrescens cv. Liangshan

作　　者：柳茜[1] 王清郦[2] 傅平[1] 孙启忠[3] 敖学成 

机构地区：[1]四川省凉山州畜科所,四川西昌615042 [2]中国农业科学院研究生院,北京100081 [3]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《草原与草坪》2013年第4期28-33,共6页Grassland and Turf

基　　金：现代农业产业技术体系(CARS-35)建设专项资助

摘　　要：对5个不同居群光叶紫花苕的种子贮藏蛋白进行SDS-PAGE比较分析。结果表明，5个居群的光叶紫花苕的盐溶蛋白和贮藏蛋白谱带表现出丰富的多态性，分子量11．09-100．09kDa。盐溶蛋白的特征蛋白谱带共有22条，多态性条带9条，多态率40．92％；贮藏蛋白的特征蛋白谱带共有31条，其中，20条为多态性条带，多态率为65．52％。利用盐溶蛋白和贮藏蛋白的条带信息进行聚类，供试材料分为2类：西昌、盐源、普格小兴场、普格居群为1类；德昌居群为另1类。表明光叶紫花苕具有较高的遗传保守性，在不同的生态区能保持较一致的遗传性。Seed storage proteins of 5 populations lyzed by proteins var. gl shared SDS-PAGE technique. The results indicated of Vicia villosa var. glabrescens cv. Liangshan were ana- that SDS-PAGE of salt-soluble proteins and seed storage were practical and reliable methods for determining relationships among 5 populations of Vicia villosa abrescens. The protein molecular bands an were shared and d 13 polymorph 11 bands were salt-soluble protei lations of Xichang lation was the oth netic conservation ban polymo ns and seed stor , Yanyuan, Puge age Xia weight ranged from 11.09 to 100.09 kDa. The salt-soluble protein had 9 ds in total 22 total bands, so the polymorphism was 40.92%. 20 bands rphism （32.14~） of total 31 seed storage protein bands. Clustering of proteins showed that 5 populations were divided into 2 groups. The oxing fie er group. The results showed Id and Puge were classified as the first group;the De that Vicia villosa var. glabresens in Liangshan area chang popu- popu- had high ge- ,which means that they had certain consistent hereditary in different ecological zones.

关 键 词：光叶紫花苕 种子贮藏蛋白 SDS-PAGE 

分 类 号：S542.035[农业科学—作物学] Q946[生物学—植物学]