血栓栓塞症患者抗活化的蛋白C的研究  

Study of APC resistance in patients with thromboembolism

作　　者：胡钧培[1] 乐忠庆[1] 熊立凡[2] 叶为德[1] 杨景文[1] 王鸿利[2] 

机构地区：[1]上海第二医科大学附属第九人民医院,上海200011 [2]上海血液学研究所

出　　处：《中国实验诊断学》2000年第5期210-212,共3页Chinese Journal of Laboratory Diagnosis

基　　金：上海教委高校科技发展基金!(96BJ01)资助

摘　　要：目的　探讨抗活化的蛋白 Ｃ（ＡＰＣＲ）现象在中国人血栓栓塞症发病中的作用。方法　用 ＡＰＴＴ法检测 ＡＰＣＲ敏感 比率（ＡＰＣＲ－ＳＲ）来研究４０例深静脉血栓症（ＤＶＴ）患者、５２例脑血栓形成患者和１００例正常人的ＡＰＣＲ发生率；用多聚酶 链反应－限制性内切酶长度多态性（ＰＣＲ－ＲＦＬＰ）来检测ＦＶ Ｌｅｉｄｅｎ突变；用免疫火箭电泳测血浆总蛋白Ｓ和游离蛋白Ｓ。结 果　正常人的正常化ＡＰＣＲ－ＳＲ（ｎ－ＡＰＣＲ－ＳＲ）为０．９３±０．３２，ＤＶＴ组和脑血栓组各有６例ｎ－ＡＰＣＲ－ＳＲ＜０．６１，ＡＰＣＲ发生率分 别为１５％和１１．５％，与正常对照组相比有显著差别；没有检测到ＦＶ Ｌｅｉｄｅｎ突变；ＤＶＴ组的蛋白Ｓ测值与正常人没有显著差 别。结论 ＡＰＣＲ可能是中国人群血栓栓塞症发病的一个危险因素，在不同种族中，致病机理不同。Objective To evaluate the effects of activated protein C resistance (APCR) in pathogenesis of thromboembolism in Chinese. Method: The incidence of APCR in 40 cases of deep venous thrombosis (DVT) patients, 52 cases of is chemic stroke and l00 normal controls were detected by activated partial thromboplastin time (APTT) in the Presence and absence of APC. FV Leiden was detected by PCR-RFLP assay.Plasma levels of TPS:Ag and FPS:Ag were measured by using rocket immunoelectrophoresis. Results: n-APCR-SR in normal controls was 0.93?.32 but there were six cases whose n-APCR-SR was below 0.61 both in 40 DVT patiods and in 52 ischemic stroke patients. The incidence of APCR in the patients was 15% and 11.5% respectively, which were significantly different from that of control group (P<0.05). No FV Leiden mutation was found in this study. There was no significant difference in Plasma PS Levels between the patients and controls. Conclusion APCR might be a risk factor in pathogenesis of thromboembolism and there existed the Plausible pathogenic divergence in the patients among different races.

关 键 词：血栓栓塞 DVT APCR APTT法 APCR-SR 

分 类 号：R446.6[医药卫生—诊断学]