γ—GTⅡ单克隆抗体在肝癌诊断中的应用  

Application of γ -GTⅡ McAb in Diagnosis of Hepatic Carcinoma.

作　　者：付平平[1] 曹纯章[1] 魏景艳[2] 

机构地区：[1]白求恩医科大学第三临床学院 [2]吉林省临床检验中心

出　　处：《中国实验诊断学》2000年第5期221-222,共2页Chinese Journal of Laboratory Diagnosis

摘　　要：目的探讨γ－谷氨酰转换酶同工酶Ⅱ（γ－ＧＴ ０Ⅱ）单克隆抗体（ＭｃＡｂ）对肝癌的诊断。方法采用ＥＬＩＳＡ法、ＡＦＰ 放射免疫法对１５４例患者血清检测。结果 ＥＬＩＳＡ法检测患者血清γ－ＧＴⅡ含量，原发性肝癌、转移性肝癌阳性率分别为 ９３％、８９％，其他肿瘤均为阴性；ＡＦＰ放射免疫法检测，原发性肝癌、转移性肝癌阳性率分别为８０．４％、５７．０％，其他肿瘤阳性率 为 ０％－１０％，病毒性肝炎、肝硬化阳性率为 ８０％以上。结论　应用 ＥＬＩＳＡ法检测血清 γ－ＧＴⅡ是目前一种诊断肝癌的非常有 价值的检测方法。Objective To study r-GTⅡ McAb in diagnosis of hepatic carcinoma. Methods Serum of 154 cases were studied by using ELISA and AFP radioimmunity. Results The positive rate of primary hepatic carinoma and metastatic hepatic carcinoma were 93% and 89%, respectively and it was zero in other carcinoma groups, by using ELISA. The positive rate of AFP was 80.4% and 57% in primary hepatic carinoma and metastatic hepatic carcinoma respectively, 10% in other carcinoma groups by AFP. The poritive rate is 86% and 80% in hepatitivirosis and cirrhosis respectively. Conclusion r- GTⅡ serum of patients was examined by using ELISA. It is a valuable method in diagnosis of hepatic carcinoma.

关 键 词：单克隆抗体 酶联免疫吸附法 肝癌 γ-GTⅡ 

分 类 号：R735.7[医药卫生—肿瘤]