单核细胞趋化蛋白-1在内源性神经干细胞迁移中的作用  

Effects of Monocyte Chemoattractant Protein-1 on Migration of Endogenous Neural Stem Cells

作　　者：丁鹏[1] 周长胜[1] 戴孝森[1] 陆斌[1] 王崇谦[1] 王伟民[1] 王进昆[1] 

机构地区：[1]昆明医科大学第一附属医院神经外科,云南昆明市650032

出　　处：《中国康复理论与实践》2013年第9期820-823,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：云南省科技厅-昆明医科大学联合专项基金(No.2010CD157)

摘　　要：目的探讨单核细胞趋化蛋白-1(MCP-1)在神经干细胞体内迁移中的作用。方法雄性Sprague-Dawley大鼠制备脑皮质微量注射脂多糖脑损伤大鼠模型,于造模后3 d、5 d、7 d灌注取脑,采用免疫组织化学方法检测MCP-1和nestin表达。结果造模后,MCP-1表达增高,5 d时达到高峰;7 d时,可在损伤区发现nestin阳性细胞。结论 MCP-1可能趋化内源性神经干细胞向脑损伤区迁移。Objective To investigate the effects of monocyte chemoattractant protein-1 （MCP-1） on the migration of neural stem cells in vivo. Methods The brain injured rat model was established with cortical micro-injection of lipopolysaccharide in Sprague-Dawley rats~ The tissue of brain was immunohistochemistrically stained to detect the expression of MCP-1 and nestin 3 d, 5 d, and 7 d after modeling. Results The expression of MCP-1 increased, and reached a peak 5 d after modeling. The nestin-positive cells was found in the lipopolysaccharide in- jected area 7 d after modeling. Conclusion MCP-1 may induce the endogenous neural stem cells migrate to lipopotysaccharide injection ar- ea in vivo.

关 键 词：脑损伤 神经干细胞 单核细胞趋化蛋白-1 迁移 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]