抗水稻白叶枯病菌植物内生真菌的筛选、鉴定  

Screening & Identification of Endophytic Fungus against Rice White Leaf Blight Xanthomonas oryzae pv. oryzae

作　　者：刘琴英[1] 谢祥聪[1] 齐育平[1] 陈璨[1] 蒋冬花[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《微生物学杂志》2013年第4期4-8,共5页Journal of Microbiology

基　　金：国家自然科学基金(31270061)

摘　　要：从多种植物叶片内分离、纯化出31株植物内生真菌,活性筛选表明分离自健康水稻叶片内生真菌Bo-1菌的乙酸乙酯粗提物对水稻白叶枯病菌具有较强的抑菌活性。当供试浓度为100μg/mL时,Bo-1菌乙酸乙酯粗提物对水稻白叶枯病菌抑菌圈为22.4 mm,与同浓度的氯霉素对水稻白叶枯病菌的抑菌效果相当。通过形态学特征观察和ITS rDNA测序分析,Bo-1菌被鉴定为淡色生赤壳菌(Bionectria ochroleuca)。抗菌谱实验表明,Bo-1菌对多种细菌类病原体具有较强的拮抗能力。31 strains were isolated and purified from endophytic fungi of various plant leaves, and activity screening suggested that ethyl acetate crude extracts of an endophytic fungal strain Bo-1 among them from healthy paddy rice leaves possessed fairly strong bacteriostatic activity against white leaf blight （ Xanthomonas oryzae pv. oryzae） （ Xoo）. When the tested concentration was at 100 μg/mL, the inhibition circle of strain Bo-1 against Xoo was 22.4 ram, which was equal to inhibition effect of chloramphenicol of the same concentration on Xoo. Strain Bo-1 was initially identified as Bionectria ochroleuca through morphological features and ITS rDNA sequence analyses. The results of antimicrobial spectrum experiments Bo-1 demonstrated that it had fairly strong antagonistic ability against various bacterial pathogens.

关 键 词：水稻白叶枯病菌 内生真菌 生赤壳菌 抑菌活性 

分 类 号：Q939.5[生物学—微生物学]