产谷氨酸脱羧酶真菌的检测和筛选  

Detection and Screening of Fungal Strains Producing Glutamic Acid Decarboxylase

作　　者：陈烟兰[1] 杨民和[1,2] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108 [2]福建师范大学工业微生物发酵技术国家地方联合工程研究中心,福建福州350108

出　　处：《微生物学杂志》2013年第4期22-29,共8页Journal of Microbiology

基　　金：福建省科技厅重点项目(2012N0013);福建省自然科学基金(2012J01122)

摘　　要：在对分离自茶叶的86个真菌菌株采用显色反应进行初选的基础上,选择谷氨酸脱羧酶(GAD)酶活相对较高的8个菌株,应用比色法和超高效液相色谱法作进一步的筛选,并对比色法和超高效液相色谱法测定GAD活性的条件进行了优化,应用优化后的方法对8个菌株产GAD能力进行了分析。结果表明在摇瓶条件下8个菌株均表现GAD活性,最高者达到43.02 U/mL。进一步超高效液相色谱法分析表明,内生真菌4号菌株GAD活性达到5.43 U/mL。为后续真菌菌株产GAD的进一步筛选和γ-氨基丁酸茶(GABA茶)的生产奠定了基础。86 fungal strains initially isolated from tea leaves were screened with chromogenic reaction method for glutamate decarboxylase （GAD） production. On this basis, 8 fungal strains of relatively higher GAD enzymatic activity were selected for further screening using colorimetry and ultra high performance liquid chromatography （UHPLC） to carry out optimization of activity conditions. The conditions of colorimetry and UHPLC for GAD activity were opti- mized. 8 strains were determined by optimized colorimetry and UHPLC. The results demonstrated that eight strains had GAD activity under the condition of shaker, the highest GAD activity reached up to 43.02 U/mL. Further analysis showed that GAD activity of endophytic fungal strain 4 was determined by UHPLC reached as high as 5.43 U/mL. The methods and conditions will be used in follow-up screening of fungal strains for the production of GABA tea.

关 键 词：GAD Γ-氨基丁酸 比色法 超高效液相色谱法 

分 类 号：Q93-31[生物学—微生物学]