MTHFR基因C667T多态性与胃癌化疗敏感的相关性探讨  

Correlation of C667T polymorphism of MTHFR gene and chemotherapy sensitivity of gastric cancer

作　　者：王文文[1] 赵蜀崖[1] 

机构地区：[1]海南省人民医院内镜室,海南海口570311

出　　处：《海南医学》2013年第18期2661-2662,共2页Hainan Medical Journal

摘　　要：目的探讨亚甲基四氢叶酸还原酶基因C667T(rs1801133)多态性与胃癌化疗敏感性的相关性。方法选择我院对5-FU敏感的胃癌患者150例作为实验组,不敏感胃癌患者120例作为对照组,利用PCR技术检测所有样本C667T位点多态性。利用统计学软件SPSS13.0对所有数据进行统计学分析。结果实验组和对照组中均检出CC、CT、TT三种基因型,三种基因型在实验组中的分布频率分别为34.0%、52.0%、14.0%,在对照组中的分布频率分别为45.0%、44.2%、10.8%。等位基因频率C/T在实验组中的分布频率为60%/40%,在对照组中的分布频率为67%/33%。统计学分析结果显示基因型在两组中的分布频率差异具有统计学意义(P<0.05)。结论 TT基因型有助于5-FU的化疗效果,而CC基因型可能是胃癌患者对5-FU化疗抵抗的重要遗传因素。Objective To investigate the correlation of C667T polymorphism of MTHFR. gene and chemo- therapy sensitivity of gastric cancer. Methods One hundred and fifty patients with gastric cancer who were sensitive to 5-FU were chosen as the experiment group, and 120 patients with gastric cancer who were not sensitivity to 5-FU were chosen as the control group. The C667T polymorphism and genotype were detected by PCR and sequencing. All the data were analyzed by SPSS 13.0. Results Three genotypes were detected, which is CC, CT and TT. The frequen- cies of CC, CT, TT were 34.0%, 52.0%, 14.0% in the experiment group and 45.0%, 44.2%, 10.8% in the control group, respectively. The allele frequency of C/T was 60%/40% in the experiment group and 67%/33% in the control group. The difference in the two group was statistically significant （P〈0.05）. Conclusion TT genotype is sensitive to the 5-FU, and the CC may be an important factor for chemotherapy resistance.

关 键 词：胃癌 亚甲基四氢叶酸还原酶基因 C667T多态性 5-氟尿嘧啶 

分 类 号：R735.2[医药卫生—肿瘤]