大肠癌组织中胃泌素与癌基因c-myc、c-fos、rasp21表达和AgNORs计数的关系  

Relationship of the expression of gastrin and oncogene c myc, c fos, ras p21 with AgNORs counting in colorectal carcinoma tissue

作　　者：何双梧[1] 何渝军[1] 沈康强[1] 陈锦[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所普通外科,重庆400042

出　　处：《第三军医大学学报》2000年第11期1065-1068,共4页Journal of Third Military Medical University

摘　　要：目的　探讨大肠癌组织中胃泌素与癌基因c myc、c fos、rasp2 1表达和核仁组成区嗜银染色 (Ag NORs)计数的关系及其意义。方法　对 48例大肠癌病人癌组织进行检测 ,采用免疫组化S P法测胃泌素、c myc、c fos、和rasp2 1表达 ,改良银染法测AgNORs计数 ,并与癌旁 3cm、6cm粘膜及正常大肠粘膜作对照。结果　癌组织胃泌素、c myc、c fos、rasp2 1表达的阳性率和AgNORs计数均显著高于癌旁 3cm、6cm粘膜和正常大肠粘膜 (P <0 .0 1) ,高分化腺癌组胃泌素表达阳性率明显高于低分化腺癌和粘液腺癌组 (P <0 .0 5 ) ,大肠癌组织胃泌素阳性组中c myc、c fos的阳性率及AgNORs计数均明显高于胃泌素阴性组 (P <0 .0 5 ,P <0 .0 1,P <0 .0 1)。结论　部分大肠癌细胞可自分泌产生胃泌素 ,介导调节c myc、c fos和rasp2 1癌基因扩增 。Objective To study the relationship of the expression of gastrin and oncogene c myc , c fos , ras p21 with AgNORs counting in colorectal carcinoma tissue and its clinical significance. Methods The expression of gastrin and c myc, c fos ras p21 of carcinoma tissue in 48 cases were detected with S P immunocytochemical method. The argyrophilia nucleolar organizer regions (AgNORs) was counted with modified Ag stain and, their expression in the mucosa 3 cm, 6 cm adjacent to carcinoma tissue and normal colorectal mucosa were also counted to be used in comparison. Results The positive expression rate of gastrin, c myc, c fos and ras p21 and AgNORs counting in carcinoma tissue were significantly higher than that in mucosa 3 cm, 6 cm adjacent to carcinoma tissue and normal colorectal mucosa( P <0.01 all). The positive expression rate of gastrin was significantly higer in highly differentiated adenocarcinoma group than in poorly differentiated and mucinous adenocarcinoma group( P <0.05). The positive expression rate of c myc and c fos and AgNORs counting were siginificantly higher in gastrin positive group than in gastrin negative group( P <0.05, P <0.01). Conclusion Certain colorectal carcinoma cells can form and secrete gastrin through autocrine and mediate the proliferation of carcinoma cells through the modulation of the amplification of oncogene c myc, c fos and ras p21.

关 键 词：大肠癌 胃泌素 癌基因 核仁组成区嗜银染色 

分 类 号：R735.340.2[医药卫生—肿瘤]