烟草PVYN辽宁分离物全序列测定与分析  

Sequencing and analysis of tobacco PVY^N Liaoning isolate

作　　者：王芳[1,2] 高正良[2] 周本国[2] 许大凤[2] 安梦楠[1] 吴元华[1] 

机构地区：[1]沈阳农业大学,植物保护学院,沈阳市110161 [2]安徽省农业科学院,烟草所,合肥市230031

出　　处：《中国烟草学报》2013年第4期109-113,共5页Acta Tabacaria Sinica

基　　金：辽宁省烟草公司科技攻关项目(辽烟计(2010)86号);安徽省烟草公司科技攻关项目(项目编号:20100551005)

摘　　要：利用引起烟草脉坏死病的马铃薯Y病毒脉坏死株系辽宁分离物(Potato virus Y—vein necrosis strain Liaoning isolate,PVYN-LN),以GenBank中已有的PVY全序列为基础,设计特异引物。通过总RNA提取、RT-PCR、3’RACE和5’RACE扩增及序列测定,测得PVYN-LN全长基因组序列(NCBI登录号为JQ971975)包括9714个碱基(含PolyA尾),整条序列仅包含一个由9186个碱基组成的开放读码框(ORF),编码一条包含3061个氨基酸的多聚蛋白。对所得序列进行BLAST,利用MEGA软件对所测得的PVYN-LN全序列与GenBank中已有的PVY全序列及氨基酸进行系统进化分析,发现PVYN-LN分离物与已报道的PVYN株系具有较高的进化亲缘关系,该分离物属于PVYN株系。序列重组分析软件RDP4分析,该序列无重组。Genome sequence of potato virus Y vein necrosis strain Liaoning isolate （PVY^LN ） from tobacco which cause tobacco vein necrosis symptom were studied. The complete genome sequence was determined to be 9,714 nucleotides in length, excluding 3 ＇ -terminal poly （A） tail, by amplification and sequencing of virus RNA. The viral genome contains a single long open reading frame of 9,186 nucleotides encoding a polyprotein of 3,061 amino acids. Complete genome sequence comparison and phylogenetic analysis indicated that pVyN-LN was most closely related to PVYN reported. It is different from other Chinese isolates and PVY isolates from other parts of the world. Analysis by sequences recombination software RDP4 showed no restructuring.

关 键 词：烟草 PVYN 基因组全序列 重组分析 

分 类 号：S572[农业科学—烟草工业]