大鼠脑梗死后脑组织TNF-α、GFAP、NSE、BDNF、Nestin的表达和神经功能变化  

The changes of nervous function and expression of TNF-α,GFAP,NSE,BDNF,and Nestin of brain tissue in rats after brain ischemia

作　　者：张昆南[1] 曹金娟[2] 刘世民[1] 胡国柱[1] 李沁妤 杨海玉[1] 吴梨华[1] 吴晓牧[1] 

机构地区：[1]江西省人民医院神经内科,江西省神经病学研究所 [2]南昌大学医学院

出　　处：《中国神经免疫学和神经病学杂志》2013年第5期305-310,共6页Chinese Journal of Neuroimmunology and Neurology

基　　金：国家自然科学基金资助项目(81160148);江西省自然科学基金资助项目(20114BAB205061);江西省主要学科学术和技术带头人培养计划(20113BCB22006);江西省科技厅基金资助项目(20132BBG70065)

摘　　要：目的探讨大鼠大脑中动脉缺血再灌注(middle cerebral artery occlusion,MCAO)模型不同时期梗死区周围组织胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)、神经元特异性烯醇化酶(neuron-specific enolase,NSE)、脑源性神经营养因子(brain derived neurotrophic factor,BDNF)、巢蛋白(Nestin)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的表达变化及其意义。方法采用线栓法制作大鼠MCAO模型,并随机分为模型1、3、7、14、21d组。采用免疫组化观察大鼠模型脑组织GFAP、NSE、BDNF、Nestin、TNF-α的表达。采用改良神经功能缺损严重程度量表(modified neurological severity score,mNSS)对MCAO模型大鼠进行神经功能评价。结果大鼠神经功能评分随MCAO术后时间延长而逐渐下降(F=121.282,P<0.05)。GFAP阳性细胞数量随MCAO术后时间推移呈双向反应,术后1、3、7d组逐渐升高,而至14d组和21d组则又逐步降低,其中7d组高于1d组、3d组(P<0.05)。NSE累积光密度(integrated optical density,IOD)随时间延长逐渐上升,其中1d组小于3d组、7d组、14d组、21d组,21d组大于3d组、7d组(P<0.05)。BDNF IOD值呈双向表达,1d组、3d组、7d组、14d组表达逐步降低,21d表达升高,其中1d组和21d组大于14d组(P<0.05)。Nestin+细胞数量随时间的推移呈双向反应,1d组～7d组逐步升高,随后逐步降低,其中7d组高于1d组、3d组(P<0.05)。MCAO后TNF-α表达无统计学差异(P=0.12)。结论 MCAO在急性期(7d前)受损脑组织产生炎症及抗炎反应明显,急性期后(7d后)以神经生长因子产生并促进神经再生为主。Objective To investigate the expression and trends of glial fibrillary acidic protein （GFAP）, neuron-specific enolase （NSE）, brain-derived neurotrophic factor （BDNF）, Nestin, tumor necrosis factor-a （TNF-a） in tissue surrounding the infarction during the acute and subacute phases after cerebral ischemia-reperfusion injury in rats. Methods Reperfusion model in rats was induced by the intraluminal suture method and randomly divided into model 1 d group, 3 d group, 7 d group, 14 d group, and 21 d group. Immunohistochemistry was used to detect the expressions of GFAP, NSE, BDNF, Nestin, and TNF-a. The mNSS was used to evaluate the neurological function of MCAO rats. Results The mNSS score gradually decreased over time after MCAO, of which the recording on the model 21 d group was significantly less than that on the model 1 d group （P〈0.05）. The numbers of GFAP positive cells appeared a two-way response with the passage of time. It gradually increased from model 1 d to 7 d group, gradually decreased at model 14 d and 21 d group. It was significantly higher at model 7 d group than that at model 1 d group and model 3 d group （P〈0. 05）. NSE integrated optical density （IOD） increased gradually. It was significantly less at model 1 d group than that at model 3 d group, 7 d group, 14 d group and 21 d group. It was significantly greater at model 21 d group than those at model 3 d group and model 7 d group （P〈0.05）. BDNF~ s IOD appeared two-way response, it began to decrease from model 1 d to 14 d group, then increased at day model 21 d group. It was significantly higher at model 1 d and 21 d group than that at model 14 d group （P〈0.05）. The amount of Nestin positive ceils presented a two-way response with the passage of time. It rose from model 1 d to 7 d group, then it gradually decreased. It was significantly greater at model 7 d group than that at model 1 d and model 3 d group （P〈0.05）. TNF-a expression had no significant difference in different MCAO model groups. Conclusi

关 键 词：脑梗死 再灌注损伤 胶质纤维酸性蛋白 神经元特异性烯醇化酶 大鼠 

分 类 号：R743.33[医药卫生—神经病学与精神病学]