非痴呆性血管性认知功能障碍患者ChAT和CHE活性变化及其临床意义  

Study of the activities of ChAT and CHE in patients with non-dementia vascular cognitive impairment

作　　者：王凡[1] 李毅[2] 肖雁[2] 徐源[1] 刘健[1] 官志忠[2] 

机构地区：[1]贵阳医学院附属医院神经外科,贵阳550004 [2]贵阳医学院分子生物学重点实验室,贵阳550004

出　　处：《重庆医学》2013年第28期3342-3343,3346,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目(81260173);贵州省科技厅基金项目(黔科合J字2012[2055])

摘　　要：目的通过检测非痴呆性血管性认知功能障碍(VCIND)患者血胆碱乙酰转移酶(ChAT)及胆碱酯酶(CHE)活性的变化,初步探讨VCIND的发生机制。方法选择61例VCIND患者作为研究组,其检测符合Rockwood认知功能障碍诊断标准但未达到NINDS-ARIIEN血管性痴呆诊断标准。同期相匹配的75例健康者作为对照组,两组均清晨空腹抽取静脉血并分离血清。应用分光光度法检测血清ChAT及CHE活性。结果血管性认知功能障碍组ChAT活性(120.94±23.93)U/mL较对照组(64.88±12.23)U/mL明显增高,差异有统计学意义(P<0.01),CHE活性两组相比差异无统计学意义(P>0.05)。结论 VCIND患者血清ChAT活性增加,促进乙酰胆碱的合成,可能是VCIND患者机体维持认知功能的一种代偿作用。Objective To study of the activities of choline acetyltransferase（ChAT） and cholinesterase（CHE） in non-dementia vascular cognitive impairment （VCIND）patients,and explore its mechanism. Methods Sixty-one patients with VCIND were select- ed as the research group （which accord with Rockwood diagnostic criteria for cognitive dysfunction but not meet the NINDS-ARI- IEN diagnostic criteria for vascular dementia）and 75 healthy people in the same period as the control group. Venous blood was cob lected in the early morning and serum was separated in both of the two groups. The activities of ChAT and CHE were detected by spectrophotometric method. Results The activity of ChAT in the research group（120. 94±23. 93） U/mL was increased significant- ly than the control group（64.88±12.23） U/mL（P〈0. 01） ,while there was no significant difference in CHE activity between the two groups（P〈0.05）. Conclusion The increased activity of ChAT in VCIND patient and enhance the synthesis of acetylcholine, which may be a compensatory mechanism of VCIND.

关 键 词：认知障碍 胆碱乙酰化酶 胆碱酯酶 

分 类 号：R749.1[医药卫生—神经病学与精神病学]