GSK-3β、PTEN、PLK1在儿童急性髓系白血病中的表达及意义  

Expression and clinical significance of GSK-3β,PTEN,PLK1 in pediatric acute myeloid leukemia

作　　者：王妍[1] 徐酉华[1] 胡艳妮[1] 孙艳辉[1] 金鑫[1] 杨桂存[1] 

机构地区：[1]重庆医科大学附属儿童医院/儿童发育疾病研究省部共建教育部重点实验室/儿科学重庆市重点实验室/重庆市儿童发育重大疾病诊治与预防国际科技合作基地,400014

出　　处：《重庆医学》2013年第28期3347-3349,共3页Chongqing medicine

摘　　要：目的探讨糖原合酶激酶-3β(GSK-3β)、与张力蛋白同源的位于第10号染色体磷酸酶基因(PTEN)、Polo样激酶1(PLK1)在儿童急性髓系白血病(AML)中的表达及意义。方法 RT-PCR方法检测实验组(33例初诊AML患儿的骨髓)和对照组(10例正常骨髓)骨髓单个核细胞(BMMNC)中GSK-3βmRNA、PTEN mRNA、PLK1mRNA的表达,ELISA方法检测GSK-3β蛋白及P-GSK-3β的表达。结果实验组中GSK-3βmRNA、GSK-3β蛋白、PLK1mRNA的表达量高于对照组(P=0.012;P=0.014;P=0.040);PTEN mRNA、P-GSK-3β的表达量低于对照组(P=0.012;P=0.002)。GSK3β蛋白与PTEN mRNA的表达呈负相关(r=-0.415,P=0.016);GSK-3βmRNA、GSK3β蛋白与PLK1mRNA的表达成呈正相关(r=0.388,P=0.026;r=0.427,P=0.013)。外周血白细胞计数增高者GSK-3β蛋白表达增高;临床危险度高者GSK-3βmRNA、GSK-3β蛋白的表达增高,P-GSK-3β的表达降低。结论在儿童AML中,GSK-3β和PLK1可能起癌基因的作用,PTEN可能起抑癌基因的作用。Objective To explore the expression and clinical significance of GSK-3β, PTEN and PLK1 in pediatric AML. Methods Experiment group was bone marrows of 33 cases incipient children with AML. Control group was 10 cases normal bone marrows. GSK-3β,PTEN and PLK1 gene expressions in BMMNC of the two groups were tested using semi quantitative reverse transcriptase polymerase chain reaction（RT-PCR）teehnique. G SK-3β protein and P-GSK-3β expressions were tested by ELISA. Re- subs The expression of GSK-3β mRNA,GSK-3β protein,PLK1 mRNA in BMMNC of children with AML was higher than that of control group（P= 0. 012 ; P = 0. 014; P = 0. 040） ; The expression of PTEN mRNA, P-GSK-3β was lower than the control group （P= 0. 012; P= 0. 002） ; GSK-3β protein had a negative correlation with PTEN mRNA（r= - 0. 415, P = 0. 016） ; GSK-3βmRNA, GSK-3β protein had a positive correlation with PLK1 mRNA（r= 0. 388, P=0. 026;r= 0. 427, P= 0. 013）. The expression of GSK-3β protein was high in which had high peripheral white blood cell counts;both the expressions of GSK-3β mRNA and GSK-3β pro- tein were high in which had high risklbut the expression of P-GSK-3βwere low. Conclusion In pediatric AML,GSK-3β and PLK1 may play a role of oncogene and PTEN may play a role of tumor suppressor gene.

关 键 词：GSK-3Β PTEN PLK1 前体细胞淋巴母细胞白血病淋巴瘤 

分 类 号：R733.7[医药卫生—肿瘤]