肾小管上皮细胞间质转分化过程中E-cadherin和α-SMA基因启动子区甲基化状态研究  

Methylation status in E-cadherin and α-SMA gene promoter region during renal tubular epithelial-mesenchymal transition

作　　者：张旭[1] 江燕[2] 张翠薇[1] 荣智利[3] 高岑[1] 马跃荣[1] 

机构地区：[1]泸州医学院病理学教研室,四川泸州646000 [2]泸州医学院附属中医院心脑病科,四川泸州646000 [3]成都医学院附属医院病理科

出　　处：《泸州医学院学报》2013年第4期328-332,共5页Journal of Luzhou Medical College

基　　金：四川省科技厅自然科学基金项目(2010JY0080)

摘　　要：目的:了解大鼠肾小管上皮细胞间质转分化与E-钙黏附蛋白(E-cadherin)和α-平滑肌肌动蛋白(α-SMA)基因启动子区甲基化状态之间的关系。方法:将大鼠肾小管上皮细胞分为对照组和TGF-β1处理组。对两组细胞采用免疫细胞化学方法检测E-cadherin和α-SMA蛋白表达情况,用ELISA法检测培养液中Ⅰ型胶原蛋白的含量。同时分别提取两组细胞中的基因组DNA,应用焦磷酸测序技术对E-cadherin和α-SMA基因启动子区甲基化水平进行检测。结果:TGF-β1处理组细胞中Ecadherin基因启动子区有2个CpG位点甲基化频率高于对照组,而α-SMA基因启动子区有2个CpG位点甲基化频率低于对照组。结论:E-cadherin和α-SMA基因启动子区甲基化状态的改变参与了肾小管上皮细胞间质转分化过程。Objective：To investigate the relationship between renal tubular epithelial-mesenchymal transition and promoter methylation status of E-cadherin and α-SMA gene. Methods： Renal tubular epithelial cells of rat were divided into control group and TGF-β1 treated group. E-cadherin and α-SMA expression were detected by immunocytochemical staining; type Ⅰ collagen concentration in culture medium was tested by ELISA method;meanwhile genomic DNA was extracted, then the E-cadherin and α-SMA promoter methylation status was detected using pyrosequencing technique. Results： There was higher methylation level and lower methylation level in promoter region ofα-SMA gene in TGF-β1 group than in control group. Conclusion：Methylation changes in E-cadherin andα-SMA gene promoter region is involved in renal tubular epithelial-mesenchymal transition.

关 键 词：E-CADHERIN α—SMA 甲基化 上皮-间质转分化 肾小管上皮细胞 

分 类 号：R365[医药卫生—病理学] R692[医药卫生—基础医学]