大鼠环孢霉素A中毒性肾损伤模型的探讨  

Establishment of nephrotoxicity model by Cyclosporin A in rats

作　　者：黄珀[1] 邓勇[2] 张春梅[1] 陈蓉[1] 张春燕[1] 万英[1] 

机构地区：[1]泸州医学院病理生理学教研室,四川泸州646000 [2]泸州医学院附属医院泌尿外科,四川泸州646000

出　　处：《泸州医学院学报》2013年第4期347-349,共3页Journal of Luzhou Medical College

摘　　要：目的:大鼠环孢霉素A中毒性肾损伤模型的探讨。方法:实验大鼠随机分为对照组,不同浓度的环孢霉素A给药组,分别给予橄榄油灌胃,不同浓度的环孢霉素A灌胃,连续4w后,测动物体重,24 h尿量,尿蛋白,取血测血肌酐、尿素氮,取肾组织作病理观察并行肾小管损伤评分,测组织超氧化物歧化酶和丙二醛含量。结果:与对照组比,25 mg/kg/d环孢霉素灌胃组肾组织结构变化明显,但功能改变不明显,50 mg/kg/d环孢霉素灌胃组肾脏形态结构及功能受损均明显,尿蛋白含量增多,血肌酐、尿素氮明显增高(P<0.05);肾组织超氧化物歧化酶含量减低而氧化产物丙二醛MDA含量明显增高(P<0.05)。结论:持续4周50 mg/kg/d剂量环孢霉素A灌胃可复制典型的环孢霉素中毒性肾损伤模型。Objective： To explore the establishment of nephrotoxicity model by Cyclosporin A（CsA）.Methods：Rats in 3 groups were treated with olive oil （control） and CsA of two different doses respectively.After 4 weeks , nephrotoxicity was assessed by the examination of body weight, serum creatinine, blood urea nitrogen, activity of antioxitant and histopathological changes. The injury of renal tubules was scored. Results： Both histopathological and functional damages were significant in CsA 50mg/kg/d group; the functional change was less conspicuous in CsA 25mg/kg/d group. Conclusion： The result indicates that a nephrotoxicity animal model can be replicated by 4 weeks intragastric administration of cyclosporine at the concentration of 50 mg/kg/d.

关 键 词：环孢霉素 肾毒性 肾损伤 

分 类 号：R363[医药卫生—病理学]