大豆GST基因家族全基因组筛选、分类和表达  

Genome-Wide Filter,Classification and Expression Analysis of GST Gene Family in Soybean

作　　者：江董丽[1] 才华[2] 端木慧子[2] 朱延明[2] 

机构地区：[1]塔里木大学生命科学学院,阿拉尔843300 [2]东北农业大学生物工程基因重点实验室,哈尔滨150030

出　　处：《分子植物育种》2013年第5期465-475,共11页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31171578);国家转基因生物新品种培育科技重大专项(2011ZX08004002-002);黑龙江省高校科技创新团队建设计划(2011TD005)共同资助

摘　　要：利用生物信息学手段,结合公共大豆基因组数据库和大豆发育表达芯片数据获得大豆GST家族基因序列、蛋白序列和染色体位置等信息并进一步对基因的组织表达等进行分析。结果显示大豆中含94个GST家族基因,根据系统发育分析将这些GST基因分成5个亚家族;定位分析表明,94个GST基因分布于大豆的16条染色体上。表达分析结果表明,14个不同发育阶段,大多成员至少在一个组织中表达,11差异表达的基因中有7在根中表达,另外4在其它部位优势表达,基因表达具有一定特异性。本研究为进一步研究GST家族的功能及其抗逆利用提供基础。Based on bioinformatic methods, soybean genome database and expression microarray data, soybean GST genes family were identified in this study. And their gene sequences, protein sequences and chromosome location were obtained. All of ninty-four GST genes from soybean were classified into 5 subfamilies according to phylogenetic relationship. And they were distributed on 16 chromosomes. Based on the soybean developmental expression database, the expression pattem of the GST gene family at different development organs and stages was analyzed. Eleven differentially expressed genes were found, and seven of them were highly expressed in root at each developmental stage by microarray. They were differentially expressed in different tissues and developmental stages. These results are helpful for their functional analysis and using for crop anti-stress improvement.

关 键 词：GST家族 全基因组筛选 分类分析 差异表达基因 

分 类 号：S565.1[农业科学—作物学]