急进高原与平原大鼠肠黏膜屏障紧密连接蛋白occludin表达的对比研究  

Expression of occludin protein in intestinal mucosa barrier in model rats of radical plateau and plain

作　　者：张方信[1] 邓芝云[2] 李文祥[1] 段云[1] 邓尚新[1] 王秀锋[1] 贾红梅[1] 马蓉霞[1] 

机构地区：[1]兰州军区兰州总医院消化内科,甘肃兰州730050 [2]兰州军区兰州总医院检验科,甘肃兰州730050

出　　处：《西北国防医学杂志》2013年第5期401-403,共3页Medical Journal of National Defending Forces in Northwest China

基　　金：全军"十一五"计划课题(06MA089);全军后勤科研计划资助项目(CLZ12J005)

摘　　要：目的:探讨急进高原与平原大鼠肠黏膜屏障紧密连接蛋白occludin在肠上皮细胞中的表达变化。方法:48只Wistar大鼠随机分为平原组(n=24)和高原缺氧组(n=24),每组再按2、4、6 d时间点随机分为3个亚组(n=8)。各组均用免疫组织化学法检测occludin蛋白表达,RT-PCR法检测occludin mRNA含量。结果:与平原组各时间点比较,高原缺氧组occludin蛋白及occludin mRNA含量显著降低(P<0.01)。结论:急进高原可使大鼠肠黏膜屏障紧密连接蛋白occludin表达减少,导致肠黏膜屏障损伤。Objective： To investigate the expression of occludin protein in intestinal mucosa barrier in model rats of radical plateau and plain. Methods： Forty - eight Wistar rats were randomly divided into plain group （ n = 24 ） and plateau group （n = 24） , then each group was randomly divided into three subgroups （n = 8 ） by 2, 4, 6 d time points. The expression of occludin protein was analyzed by immunohistochemistry, and the content of occludin mR- NA was tested by RT -PCR. Results： At each time point, occludin protein and occludin mRNA in plateau group were significantly lower than that in plain group （P 〈 0.01 ）. Conclusion： The expression of occludin protein in intestinal mucosa barrier decreased in model rats of radical plateau, which may lead to the intestinal mucosa barrier damaged.

关 键 词：肠黏膜屏障 高原 缺氧 OCCLUDIN蛋白 

分 类 号：R363[医药卫生—病理学]