甜菜碱对戊四氮致痫大鼠海马GFAP、甘氨酸和甘氨酸受体表达的影响  

Effects of betaine on expression of GFAP,glycine and glycine receptor in hippocampus of pentylenetetrazole-induced epileptic rats

作　　者：王辉[1] 王淑湘[1] 崔国利[2] 卢春凤[1] 刘蕾[1] 刘君星[1] 马小茹[1] 王芳芳[1] 梁衍锋[1] 吴佳梅[1] 毛金军[1] 王淑秋[1] 

机构地区：[1]佳木斯大学基础医学院,黑龙江佳木斯154007 [2]佳木斯大学第一附属医院检验科,黑龙江佳木斯154002

出　　处：《中国病理生理杂志》2013年第9期1657-1661,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.81241112);黑龙江省研究生创新基金资助项目(No.YJSCX2011-375HLJ);黑龙江教育厅科学技术研究项目(No.11521276)

摘　　要：目的:研究甜菜碱对癫痫大鼠海马胶质细胞原纤维酸性蛋白(GFAP)、甘氨酸(Gly)及甘氨酸受体(GlyR)表达的影响。方法:将健康雄性Wistar大鼠随机分为:正常对照组(腹腔注射生理盐水,1.0 mL生理盐水灌胃);癫痫组(腹腔注射戊四氮,1.0 mL生理盐水灌胃);甜菜碱高、中、低浓度组(腹腔注射戊四氮,甜菜碱灌胃);丙戊酸钠组(腹腔注射戊四氮,丙戊酸钠灌胃)。实验结束后大鼠眼眶取血检测血清中同型半胱氨酸的含量;在低温条件下迅速取脑组织,分析Gly含量的变化,免疫荧光检测GFAP的水平,兔疫荧光和Western bloting检测海马区GlyR表达的变化。结果:各组大鼠大发作潜伏期无显著差异(P>0.05),但是甜菜碱治疗组较癫痫组的首次大发作持续时间显著缩短(P<0.01)。癫痫组同型半胱氨酸的含量与正常组比较显著降低(P<0.01),甜菜碱高、低浓度组同型半胱氨酸含量与癫痫组比较明显降低(P<0.05)。癫痫组甘氨酸的含量与正常对照组相比显著下降(P<0.01)。甜菜碱高、中、低浓度组甘氨酸的含量与癫痫组比较显著增高(P<0.01)。免疫荧光检测GFAP结果,癫痫组与正常组比较显著增高(P<0.01),而甜菜碱高中低浓度与癫痫组相比显著降低(P<0.01)。免疫荧光与Western bloting检测GlyR结果,癫痫组GluR的表达与正常对照组相比显著降低(P<0.01),甜菜碱高、中、浓度组较癫痫组显著升高(P<0.01)。结论:甜菜碱具有较好的抗癫痫作用。AIM：To study the effects of betaine on glial fibrillary acidic protein （GFAP）, glycine （Gly） and glycine receptor （GlyR） expression in the hippocampus of rats with epilepsy induced by pentylenetetrazole （PTZ）. METHODS：Forty-eight healthy male Wistar rats were randomly divided into control group, PTZ （35 mg·kg-1·d-1, intraperitoneal injection） group, PTZ＋betaine （450 mg·kg-1·d-1, intragastric administration） group, PTZ＋betaine （225 mg·kg-1·d-1, intragastric administration） group, PTZ＋betaine （112.5 mg·kg-1·d-1, intragastric administration） group and PTZ＋sodium valproate （200 mg·kg-1·d-1, intragastric administration） group. The rats in control group were intraperitoneally injected with saline at the same volume as PTZ injection, and those in control group and PTZ group received intragastric administration of saline at 1.0 mL·d-1. Rat behavior was recorded. Serum homocysteine （Hcy） level was measured. The expression of GFAP in the hippocampus was measured by immunofluorescence. Hippocampal Gly content was measured by an amino acid analysis system. The expression of GlyR was detected by immunofluorescence and Western blotting. RESULTS：There was no difference in the latency of grand mal seizures among groups （P〉0.05）. However, betaine treatment significantly decreased the duration of the first grand mal seizure compared with PTZ group （P〈0.01）. Serum Hcy level in PTZ group was significantly lowered compared with control group （P〈0.01）, and further decreased after betaine treatment （P〈0.05）. GFAP in PTZ group was significantly higher than that in control group （P〈0.01）, and decreased after betaine treatment （P〈0.05）. Gly in PTZ group was significantly lowered compared with control group （P〈0.01）, and increased after betaine treatment （P〈0.05）. The content of GlyR among groups showed the same trend as Gly. CONCLUSION：Betaine treatment shows antiepileptic effect, which may be related to its effects on the me

关 键 词：甜菜碱 癫痫 同型半胱氨酸 胶质细胞原纤维酸性蛋白 甘氨酸 甘氨酸受体 

分 类 号：R363[医药卫生—病理学]