恶性胸水中醛缩酶A的水平及其对人肺腺癌细胞增殖、迁移与侵袭能力的影响  

Aldolase A level in malignant pleural effusion and its effects on proliferation,migration and invasion of human lung adenocarcinoma cells

作　　者：冯小鹏[1,2] 孙珍贵[1] 汪向海[1] 邢敏[1] 陈兴无[1] 

机构地区：[1]皖南医学院弋矶山医院呼吸内科,安徽芜湖241001 [2]广东省惠州市第一人民医院重症医学科

出　　处：《中国病理生理杂志》2013年第9期1662-1667,共6页Chinese Journal of Pathophysiology

基　　金：弋矶山医院引进人才专项科研基金资助项目(No.YR201012)

摘　　要：目的:比较肺癌患者伴恶性胸水(MPE)和结核性胸膜炎患者胸水(TBPE)中醛缩酶A(ALDOA)的表达水平及其与癌胚抗原(CEA)和乳酸脱氢酶(LDH)的相关性,探讨ALDOA对肺腺癌A549细胞增殖、迁移及侵袭的影响。方法:ELISA和化学发光法检测65例MPE和35例TBPE中ALDOA、CEA和LDH水平;以不同浓度的ALDOA作用于A549细胞,分别采用MTT法、划痕及体外侵袭实验研究ALDOA对A549细胞增殖、迁移和侵袭的影响。结果:MPE中ALDOA浓度[(46.8±21.4)μg/L]明显高于TBPE[(23.9±17.2)μg/L](P<0.01),其中腺癌[(71.7±32.1)μg/L]明显高于鳞癌[(21.3±14.6)μg/L](P<0.05);MPE中CEA和LDH水平[(82.2±56.6)μg/L和(755.8±382.5)U/L]也明显高于TBPE[(12.6±9.7)μg/L和(388.4±163.9)U/L](P<0.01)。2组患者胸水中ALDOA与CEA和LDH均呈正相关关系(P<0.01或P<0.05)。不同浓度ALDOA刺激A549细胞后,细胞增殖增强且迁移、侵袭加快,并呈浓度依赖性。结论:肺癌患者MPE中ALDOA表达水平明显高于TBPE中的水平,其中肺腺癌胸水ALDOA水平明显高于鳞癌;ALDOA与CEA和LDH水平高度正相关;ALDOA浓度依赖性地增强肺腺癌A549细胞增殖、迁移和侵袭。AIM： To investigate the levels of aldolase A （ALDOA）, carcinoembryonic antigen （CEA） and lactate dehydrogenase （LDH） in malignant pleural effusion （MPE） from patients with lung cancer and tuberculous pleural effusion （TBPE） from patients with tuberculous pleurisy, and to explore the effects of ALDOA on the proliferation, migration and invasion of human lung adenocarcinoma A549 cells. METHODS：Pleural effusion samples including 65 cases of MPE and 35 cases of TBPE were collected, and the levels of ALDOA, CEA and LDH were detected by ELISA and chemiluminescence assay. After A549 cells were treated with different concentrations of ALDOA, the proliferation, migration and invasion of the cells were investigated by MTT assay, scratch test, Matrigel assay and Transwell invasion assay. RESULTS：The levels of ALDOA, CEA and LDH in MPE were （46.8±21.4） μg/L, （82.2±56.6） μg/L and （755.8±382.5） U/L, respectively, which were significantly higher than those in TBPE [（23.9±17.2） μg/L, （12.6±9.7） μg/L and （388.4±163.9） U/L, respectively; P〈0.01]. The concentration of ALDOA in MPE from adenocarcinoma patients [（71.7±32.1） μg/L] was significantly higher than that in MPE from squamous-cell carcinoma patients [（21.3±14.6） μg/L, P〈0.05]. The concentrations of ALDOA in MPE and TBPE were positively correlated with the concentrations of CEA and LDH （P〈0.01 or P〈0.05）. ALDOA enhanced the proliferation, migration and invasion of A549 cells in a concentration-dependent manner. CONCLUSION：The expression level of ALDOA in MPE is significantly higher than that in TBPE, especially in MPE from lung adenocarcinoma patients. There are highly positive correlations between ALDOA and CEA, ALDOA and LDH in pleural effusion. ALDOA concentration-dependently promotes the proliferation, migration and invasion of A549 cells.

关 键 词：醛缩酶A 胸水 肺肿瘤 细胞增殖 细胞迁移 肿瘤侵袭 

分 类 号：R363[医药卫生—病理学]