心肌细胞培养上清诱导骨髓间充质干细胞的分化  

Supernatant of myocardiocyte induces differentiation of bone marrow-derived mesenchymal stem cells

作　　者：李春梅[1] 王秀力[1] 朱海亮[2] 王洁[1] 宫海滨[1] 

机构地区：[1]徐州市心血管病研究所,徐州市中心医院,江苏省徐州市221009 [2]南京大学生命科学学院,江苏省南京市210093

出　　处：《中国组织工程研究》2013年第36期6417-6422,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30572073);江苏省自然科学基金项目(DK2005428);江苏省卫生厅重大项目(K200402);江苏省医学重点人才(RC2007024);徐州市科技发展项目(XF10C029)~~

摘　　要：背景:前期研究发现,心肌细胞培养上清具有诱导骨髓间充质干细胞分化为心肌样细胞的能力,这可能与培养上清内的某种细胞因子或几种细胞因子有关。目的:分析大鼠心肌细胞培养上清诱导骨髓间充质干细胞分化为心肌样细胞是否与心肌细胞培养上清内细胞因子含量不同有关。方法:采用全骨髓贴壁培养法分离骨髓间充质干细胞并在体外培养纯化,酶消化法分离心肌细胞,分别以1×108L-1的细胞浓度培养72 h后收集上清。用酶联免疫吸附试验技术检测心肌细胞和骨髓间充质干细胞培养上清内的肝细胞生长因子、胰岛素样生长因子1、血小板源生长因子、干细胞因子、成纤维细胞生长因子和血管内皮生长因子的水平。结果与结论:心肌细胞培养上清组内的胰岛素样生长因子1、血小板源生长因子和成纤维细胞生长因子水平明显高于骨髓间充质干细胞培养上清组(P<0.01),提示心肌细胞培养上清内的胰岛素样生长因子1、血小板源生长因子和成纤维细胞生长因子可能与诱导骨髓间充质干细胞分化为心肌样细胞有关,其中主要的细胞因子是胰岛素样生长因子1。BACKGROUND： Culture supernatant containing myocardiocyte has been demonstrated to induce differentiation of bone marrow-derived mesenchymal stem cells into myocardiocyte-like cells. This may associate with some or several cytokines in the culture supernatant. OBJECTIVE： To explore if the supernatant of myocardiocyte induces bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells is associated with the different cytokine content in the supernatant of myocardiocyte. METHODS： Bone marrow-derived mesenchymal stem cells were isolated and cultured in vitro by the whole bone marrow adherent culture. Cardiocytes were isolated and cultured by enzyme digestion. Bone marrow-derived mesenchymal stem cells （1×10^8/L） and cardiocytes （1×10^8/L） were cultured for 72 hours and the supernatant was collected. Hepatocyte growth factor, insulin-like growth factor 1, platelet-derived growth factor, stem cell factor,fibroblast growth factor and vascular endothelial growth factor levels in culture supernatant of bone marrow-derived mesenchymal stem cells and cardiocytes were detected by enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION： The contentof insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes was significantly higher in cardiocytes group compared with bone marrow-derived mesenchymal stem cells group （P 〈 0.01 ）. Results indicated that insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes may have capability to induce bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells, and insulin-like growth factor 1 may serve as the main cytokine.

关 键 词：干细胞 间质干细胞 肌细胞 心脏 胰岛素样生长因子Ⅰ 血小板源性生长因子 肝细胞生长因子 

分 类 号：R394.2[医药卫生—医学遗传学]