高含量DHA/EPA甘油三酯对脂质代谢相关酶活力的影响  

Effects of high content of DHA/EPA-triglyceride on the activities of enzymes related to Lipid metabolism

作　　者：朱昱哲[1] 王静凤[1] 张维刚[1] 孙兆敏[1] 王玉明[1] 薛长湖[1] 

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《中国海洋药物》2013年第5期33-38,共6页Chinese Journal of Marine Drugs

基　　金：山东省科技发展计划(2012G0021506);海洋公益性行业科研专项(201105029);山东省自主创新专项(2012CX80201);长江学者和创新团队发展计划资助(IRT1188)资助

摘　　要：目的研究高含量DHA/EPA甘油三酯对脂肪肝模型大鼠肝脏脂质代谢相关酶活力的影响。方法采用1%乳清酸饲喂法建立脂肪肝大鼠模型。灌胃高含量DHA/EPA甘油三酯20d后,检测大鼠血清和肝脏总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)含量以及肝脏脂肪合成和分解相关酶活力。结果高含量DHA/EPA甘油三酯可预防大鼠血清和肝脏脂质含量升高(P<0.05,P<0.01),有效降低脂肪酸合成酶(fatty acid synthesase,FAS)、苹果酸酶(malic enzyme,ME)等脂肪合成酶活力(P<0.01),显著提高肉碱棕榈酰转移酶(carnitine palmitoyl transterase-1,CPT-1)和过氧化物酶体β-氧化酶系(peroxisomalβ-oxidation enzymes,POE)等脂肪分解酶活力(P<0.01),其效果明显优于乙酯型和低含量DHA/EPA甘油三酯型鱼油。结论高含量DHA/EPA甘油三酯通过抑制脂肪合成,促进脂肪氧化分解,有效调节脂肪肝大鼠的脂质代谢。Objective To investigate the effects of high content of DHA/EPA-triglyceride on the activities of enzymes related to lipid metabolism in fatty liver rats. Methods The fatty liver rats model was in- duced by feeding AIN93 rats with 1% orotic acid. The serum and hepatic lipids concentrations and the activities of enzymes related to Lipid metabolism were detected after given high content of DHA/EPA- triglyceride for 20 days. Results High content of DHA/EPA-triglyceride could prevent the increase of TC and TG in serum and liver（P〈0. 05,P〈0. 01）; decrease the activity of FAS and ME（P〈0. 01）, and enhance the activity of CPT-1 and POE（P〈0. 01）, The effect of high content of DHA/EPA-tri- glyceride was significantly better than the effects of ethyl-DHA/EPA-triglyceride as well as the effect of low content of DHA/EPA-triglyceride fish oils. Conclusion High content of DHA/EPA-triglyceride could improve lipid metabolism in fatty liver rats by inhibiting the synthesis of lipids and enhancing its catabolism.

关 键 词：乳清酸 脂质代谢 DHA EPA甘油三酯 FAS ME CPT-1 POE 

分 类 号：R965[医药卫生—药理学]