宁波沿海陆源排污口假单胞菌属(Pseudomonas)分布特点  

DISTRIBUTION CHARACTERISTICS OF PSEUDOMONAS IN NINGBO COASTAL SEWAGE OUTLETS

作　　者：陈丽萍[1] 侯付景 张迪骏[1] 何伟娜[1] 周君[1] 张春丹[1] 童茜茜[1] 王中华[1] 李太武[3] 苏秀榕[1] 

机构地区：[1]宁波大学海洋学院,宁波315211 [2]宁波绿之健药业有限公司,宁波315505 [3]宁波城市职业技术学院,宁波315100

出　　处：《海洋与湖沼》2013年第4期926-930,共5页Oceanologia Et Limnologia Sinica

基　　金：海洋公益性行业专项经费资助项目;201105007-4号;浙江省重大科技攻关项目;2006C13089号;宁波市科技局资助项目;2008C50027号

摘　　要：采用高通量454焦磷酸测序方法对宁波沿海2个重点排污口、8个一般排污口的20个站位水样进行分析,得到2011年3月、5月、8月、10月份各排污口假单胞菌属的分布情况。结果表明,宁波沿海陆源排污口中存在较多的假单胞菌属。在检出的假单胞菌属中,维罗纳假单胞菌(Pseudomonas veronii)和莓实假单胞菌(Pseudomonas fragi)的含量相对较多,分别占56.72%、13.904%;排污口中假单胞菌属的数量存在季节性差异,3月份、5月份假单胞菌的数量相对较多,8月份和10月份较少,推测与季节性温度变化有关;假单胞菌属的含量与排污口的主要污染物有关,氨氮含量较高的排污口假单胞菌属的含量更高。Water samples were collected at 20 sites spread across 10 sewage outlets, two key sewage outlets, and eight general sewage outlets in Ningbo City during March, May, August, and October of 2011. Samples were analyzed using high-throughput 454 pyrophosphate sequencing to determine the distribution of pseudomonas. The results show pseudomonas was present in sewage off the coast. pseudomonas veronii and pseudomonas fragi were the most common species, accounting for 56.72% and 13.90%, respectively, of all detectable pseudomonas. The numbers of pseudomonas showed seasonal differences, more in March and May, and less in August and October, indicating seasonal alternation in temperature. The numbers of pseudomonas were associate with the main pollutant in the outlets, and higher levels of ammonia nitrogen corresponding to more pseudomonas.

关 键 词：假单胞菌属 454焦磷酸测序 陆源排污口 聚类分析 

分 类 号：X511[环境科学与工程—环境工程]