胡桃醌对人前列腺癌PC-3细胞抑制作用  

Inhibitive effect of juglone on prostate cancer PC-3 cells

作　　者：方芳[1] 王立国[1] 张巍[1] 陈艳媛[1] 杨冬梅[1] 王宇[1] 

机构地区：[1]吉林医药学院病原学教研室,吉林吉林132013

出　　处：《中国公共卫生》2013年第10期1458-1460,共3页Chinese Journal of Public Health

基　　金：吉林省教育厅"十二五"教育技术研究项目(吉教科合字2011第286号);吉林省教育厅"十二五"教育技术研究项目(吉教科合字2012第333号)

摘　　要：目的探讨胡桃醌对前列腺癌PC-3细胞的抑制作用。方法溴化四氮唑蓝(MTT)法检测不同剂量胡桃醌对PC-3细胞生长抑制影响;采用Annexin V-FITC/PI染色实验,流式细胞仪检测PC-3细胞凋亡情况;蛋白印迹法检测B细胞淋巴瘤2(Bcl-2)蛋白和Bcl-2相关X蛋白(Bax)的表达量。结果与对照组比较,12.5、25、50和100μmol/L胡桃醌对前列腺癌PC-3细胞具有抑制作用且呈剂量依赖性,抑制率分别为20.6%、39.7%、57.3%和66.2%;流式细胞仪检测胡桃醌(≥25μmol/L)作用细胞后诱导PC-3细胞发生早期和晚期凋亡,当胡桃醌浓度为25、50和100μmol/L时其早、晚期凋亡率分别是7.37%和2.07%、11.03%和5.8%及18.62%和8.54%,均高于对照组诱导的细胞凋亡率(P<0.05);随着胡桃醌浓度(≥50μmol/L)不断增加,可上调Bax并下调Bcl-2蛋白的表达(P<0.05)。结论胡桃醌对前列腺癌PC-3细胞具有细胞毒作用,抑制细胞生长。Objective To explore inhibitive effect of juglone on prostate cancer PC-3 cells.Methods The 3-（4,5 dimethyl-2-thiazolyl）-2,5-diphenyl-2H-tetrazolium bromide（MTT）assay was used to determine the growth of PC-3 cells treated with different concentrations of juglone.The induction of apoptosis was detected by flow cytometry with annexin V-FITC/PI staining.Western blot was used to detect the expression of apoptosis-associated protein of B-cell lymphoma（Bcl-2）and Bcl-2-associated X protein（Bax）.Results Juglone could significantly inhibit the growth of PC-3 cells in dose-dependent manner with the inhibition ratios of 20.6%,39.7%,57.3%,and 66.2%,respectively.Flow cytometry detection showed that the early and late apoptosis rates of PC-3 cells treated with juglone of 25,50,and 100 μmol/L were 7.37% and 2.07%,11.03% and 5.8%,and 18.62% and 8.54%,respectively,and higher than those of the control group（P〈0.05）.High dose of juglone（≥50 μmol/L）could reduce the expression of apoptosis-regulated protein Bcl-2 and increase the expression of apoptosis-regulated protein Bax（P〈0.05）.Conclusion Juglone can suppress the growth of PC-3 prostate cancer cells.

关 键 词：胡桃醌 PC-3细胞 凋亡 

分 类 号：R730[医药卫生—肿瘤] R737.25[医药卫生—临床医学]