肝纤维化大鼠肝脏ACE/ACE2的动态变化  

作　　者：王晋江[1] 霍丽娟[1] 武淑君[1] 张婕[1] 贾慧[1] 

机构地区：[1]山西医科大学附属第一临床医学院消化科,山西030001

出　　处：《当代医学》2013年第28期22-24,共3页Contemporary Medicine

摘　　要：目的研究肝纤维化进程中大鼠肝脏血管紧张素转换酶(ACE)、血管紧张素转换酶(2ACE 2)水平及ACE/ACE 2的动态变化。方法制备四氯化碳(CCl4)诱导大鼠肝纤维化模型,分别于造模第15、30、45、60天处死模型组大鼠各6只,各组大鼠肝组织行HE和Masson染色,Western blot法检测肝组织匀浆ACE、ACE2蛋白表达。结果在大鼠肝纤维化过程中,肝组织ACE、ACE 2水平呈上升趋势;第15、30天模型组ACE/ACE 2比值较对照组降低(P<0.05),而第45天模型组其比值高于对照组(P<0.05),第60天时该比值明显增高(P<0.01)。结论 ACE、ACE 2均参与大鼠肝纤维化形成。Objective To explore variation on level of angiotensin converting enzyme（ACE） and angiotensin converting enzyme 2（ACE 2）, ratio of ACE/ACE 2 in hepatic fibrosis in rats. Methods Except rats in control group,all were given subcutaneous injection of 40% CC14. Each 6 rats in the model group were killed at 15, 30, 45 and 60 day respectively. Histopathological study of liver tissue was done with hematoxylin-eosin（HE）and Masson trichrome staining.ACE arid ACE 2 levels were determined by Western blot analysis. Results The level of ACE and ACE 2 show ascendant trend during fibrosis rats, ACE^ACE 2 ratio of model group at 15, 30 day reduced than control group. Conclusion The occurrence and development of hepatic fibrosis are possibly associated with imbalances between ACE and ACE 2.

关 键 词：肝纤维化 血管紧张素转换酶 血管紧张素转换酶2 

分 类 号：R575.2[医药卫生—消化系统]