影响小鼠胚胎显微注射成功率的若干因素  

SOME FACTORS AFFECTING MICROINJECTION SUCCESS RATE IN THE PRODUCTION OF TRANSGENIC MICE

作　　者：张德福[1] 陈茵[2] 王英[1] 王凯 张平[1] 李震[1] 

机构地区：[1]上海市农业科学院畜牧兽医研究所，上海 201106 [2]上海第二医科大学附属上海新华医院，上海 200836 [3]上海转基因研究中心，上海 200433

出　　处：《上海农业学报》2000年第4期57-59,共3页Acta Agriculturae Shanghai

基　　金：上海市自然科学基金资助

摘　　要：显微注射法是目前国际公认的制备转基因动物的首选方法 ,有重大的应用价值 ,影响小鼠显微注射成功有许多因素。本文根据我们显微注射的工作 ,着重探讨影响小鼠显微注射成功的受精卵的质、量和原核清晰度三个关键因素。Microinjection is the most efficient and practical method to produce transgenic animal to date. There are many factors affecting microinjection success rate in the production of transgenic mice. According to the microinjection works in our laboratory, we studied emphaticallty three key factors affecting microinjection success rate in the production of transgenic mice-quantity, quality of fertilized eggs and definition of pronuclei.

关 键 词：显微注射 受精卵 原核 转基因动物 成功率 

分 类 号：S814.8[农业科学—畜牧学]