蛋白质SDS-PAGE用于螺旋藻分类及突变体鉴定的研究  

Studies on taxonomy and mutants identification by protein SDS PAGE in Spirulina

作　　者：汪志平[1] 

机构地区：[1]浙江大学原子核农业科学研究所，浙江 杭州 310029

出　　处：《浙江大学学报（农业与生命科学版）》2000年第6期583-587,共5页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30000010) ;浙江省科委"九五"重点资助项目 (961102208-001)

摘　　要：螺旋藻经 SDS提取液提取、冷丙酮沉降后 ,所得蛋白液的 SDS- PAGE图谱条带清晰、信息量大 .对 10株钝顶螺旋藻 ( Spirulina platensis) Sp- D,Sp- Y,Sp- F,Sp- J,Sp- B,Sp- S,Sp- K,Sp- Z及其突变体 Sp- Z( S)和 Sp- Z( L) ,所作的蛋白质 SDS- PAGE分析表明 :1螺旋藻蛋白带主要分布于 31k D和188k D之间 ,各藻株间的蛋白质电泳图谱的特征性差异可作为螺旋藻分类的主要依据之一 ;2 Sp- J和Sp- K的蛋白质 SDS- PAGE图谱相同 ,从而进一步确证它们属同一品系 ;3 Sp- Z( S)和 Sp- Z( L )作为Sp- Z的耐盐突变体和直线形突变体 ,分别比 Sp- Z多 1条 4 3k D及少 1条 87k D的蛋白带 ;4所建立的蛋白质 SDS-The SDS PAGE patterns of proteins, which extracted from Spirulina platensis by extractant containing SDS and purified by -20℃ actone,were clear and had many bands.The SDS PAGE analysis of Sp D,Sp Y,Sp F,Sp J,Sp B,Sp S,Sp K,Sp Z and it’s mutants:Sp Z(S) and Sp Z(L) showed that: (1) The protein bands of Spirulina mainly distributed from 31 kD to 188 kD,the patterns among strains were similar,but there were some remarkable differences,which could be valuable in the systematic taxonomic study of Spirulina ; (2) The same protein patterns of Sp J and Sp K further indicated that they belonged to same strain; (3) Sp Z(S) and Sp Z(L) are the mutants from Sp Z. A new 43 kD protein appeared on the pattern of Sp Z(S) which could grow splendidly in sea water media.And the 87 kD protein disappeared on the pattern of Sp Z(L) with linear filaments; (4) The methods of protein SDS PAGE could be useful in the taxonomic study and mutant identification of Spirulina .

关 键 词：螺旋藻 蛋白质 分类 突变体鉴定 SDS-PAGE 

分 类 号：Q949.222[生物学—植物学]