罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭的影响  

Effect of basil polysaccharide on the invasiveness of M DA-M B-231 cells

作　　者：石焕[1,2] 邵倩倩[1] 孙锦堂[1] 宋丙凤[1] 吕静[1,3] 何影[1] 张韵[1] 曲迅[1] 

机构地区：[1]山东大学齐鲁医院临床基础研究所,济南250012 [2]山东省肿瘤医院内科三病区,济南250117 [3]青岛大学医学院附属医院肿瘤科,山东青岛266071

出　　处：《山东大学学报（医学版）》2013年第9期50-54,共5页Journal of Shandong University:Health Sciences

摘　　要：目的观察罗勒多糖对乳腺癌细胞系MDA-MB-231侵袭性的影响。方法将体外培养的MDA-MB-231细胞分为实验组和对照组。实验组细胞用不同浓度罗勒多糖(100、300μg/mL)处理,对照组不加任何刺激因子。Transw ell小室检测细胞侵袭能力,RT-PCR、Western blotting方法检测与侵袭相关的基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)和膜型基质金属蛋白酶1(MT1-MMP)的表达。结果①罗勒多糖可抑制MDA-MB-231细胞的侵袭力(P<0.01);②罗勒多糖抑制MT1-MMP分子的mRNA表达(P<0.05)及下调其蛋白表达水平。③罗勒多糖对MMP-2及MMP-9分子的mRNA表达无影响。结论罗勒多糖可通过下调MT1-MMP的表达抑制MDA-MB-231细胞的侵袭。Objective To investigate the effect of basil polysaccharide on the invasiveness of MDA-MB-231 cells. Methods MDA-MB-231 cells cultured in vitro were divided into the experimental group and control group. The con- trol group was not treated, while the experimental group was treated with 100 p^g/mL or 300 ~g/mL basil polysaccha- ride. The invasiveness of the cells was detected with transwell assay. The expressions of matrix metalloproteinase-2 （ MMP-2）, matrix metalloproteinase-9 （MMP-9） and membrane-type 1 matrix metalloproteinase （MT1-MMP） were tested with RT-PCR and Western blotting. Results Basil polysaccharide suppressed the invasion of MDA-MB-231 cells （P 〈 0.01 ）, decreased the mRNA expression of MT1-MMP （P 〈 0.05 ）, but had no effect on the mRNA expres- sion of MMP-2 and MMP-9. Conclusion Basil polysaccharide inhibits the invasiveness of MDA-MB-231 cells by re- ducing the expression of MT1-MMP.

关 键 词：罗勒多糖 肿瘤侵袭 膜型基质金属蛋白酶-1 乳腺肿瘤 

分 类 号：R73-361[医药卫生—肿瘤]