藤黄酸诱导人肝癌细胞株Bel-7402凋亡  

Gambogic acid induces apoptosis of human liver cancer Bel-7402 cell line

作　　者：顾炎[1] 姜晓荣[2] 

机构地区：[1]东南大学附属南京市第二医院肿瘤科,江苏省210003 [2]河海大学医院

出　　处：《江苏医药》2013年第17期1999-2002,共4页Jiangsu Medical Journal

摘　　要：目的探讨藤黄酸(GA)对人肝癌Bel-7402细胞的诱导凋亡和增殖抑制作用。方法采用四甲基偶氮唑蓝(MTT)比色法检测细胞生长的抑制作用,流式细胞术检测细胞凋亡率及细胞周期变化,RT-PCR和Western blot分别检测Bcl-2、Bax mRNA及蛋白质的表达量。结果经1、2、4μmol/L GA作用Bel-7402细胞24、48、72h后,细胞凋亡率有不同程度上升,呈现剂量和时间依赖性细胞增殖抑制作用;细胞凋亡率随着GA浓度的增加而逐步增高,且产生G2/M期细胞周期阻滞和凋亡;Bax mRNA和蛋白表达上调,而Bcl-2mRNA及蛋白表达降低。结论 GA对Bel-7402细胞有显著的诱导凋亡及增殖抑制作用。其作用机制可能与上调Bax基因、下调Bcl-2基因的表达以及对细胞周期的阻滞有关。Objective To investigate the proliferation inhibition and apoptosis of human liver cancer Bel-7402 cell line induced by GA in vetro. Methods The cell proliferation was determined by MTT assay. The cell apoptosis and cell cycle were analyzed by flow cytometry, The expressions of Bcb2 and Pax mRNA and protein were detected by RT PCR and Western blot, respectively. Results GA inhibited the proliferation of Bel-7402 cells in the does- and time-dependent manners. As the concentration of GA increased, the apoptosis rate of the cells increased. Meanwhile, GA induced 02/M cell cycle arrest. The expressions of Pax mRNA and protein were upregulated while those of BcL2 were downregulated. Conclusion GA inhibits cell proliferation and enhances cell apoptosis, which may be related to the upregulation of Bax gene expression, downregulation of Bcl-2 gene expression and the cell cycle arrest.

关 键 词：藤黄酸 Bel-7402细胞株 BAX BCL-2 

分 类 号：R735[医药卫生—肿瘤]