骨形态发生蛋白9对食管鳞状细胞癌细胞的抑制作用  

Inhibitory effect of bone morphogenetic protein 9 on esophageal squamous cancer cells

作　　者：胡敏[1] 宋培培[1] 湛晓琴[1] 吕自兰[1] 章帆[1] 翁亚光[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室重庆市重点实验室,重庆400016

出　　处：《中国生物制品学杂志》2013年第9期1237-1241,共5页Chinese Journal of Biologicals

基　　金：教育部高等学校博士学科点专项科研基金(20115503110009)

摘　　要：目的 探讨骨形态发生蛋白9（Bone morphogenetic proteins 9,BMP 9）对食管鳞状细胞癌细胞增殖、克隆、迁移、侵袭及细胞周期的影响。方法 用AdGFP和AdBMP 9分别感染3株食管鳞状细胞癌细胞株ECA109、KYSE150和KYSE180,并设不感染病毒的3种细胞作为对照,采用RT-PCR法检测AdBMP 9感染的3株细胞中BMP 9基因mRNA的转录水平,MTT法检测病毒感染细胞0～96h各组细胞的增殖活力,平板克隆法检测各组细胞的克隆形成能力,划痕试验检测各组细胞的迁移能力,Transwell小室试验检测各组细胞的侵袭能力,并对3组ECA109细胞进行细胞周期的检测。结果 AdBMP 9感染的3株细胞中BMP 9基因mRNA的转录水平均明显提高。与AdGFP感染和未感染病毒的细胞相比,3种细胞在过表达BMP 9后,增殖活力明显降低（P〈0.05）;细胞克隆形成能力均下降（下降率达70%以上）,且克隆细胞团明显变小;细胞迁移和侵袭能力均下降;AdBMP 9感染的ECA109细胞处于G1期的细胞比例明显上升,S期比例明显下降（P〈0.05）。结论 BMP 9可明显抑制食管鳞癌细胞的增殖、克隆、迁移和侵袭能力,并将细胞周期阻滞于G1期。Objective To investigate the effect of bone morphogenetic protein 9 （BMP9） on proliferation, cloning, migration, invasion and cell cycle of esophageal squamous cancer cells. Methods Three esophageal squamous cancer cell lines, ECA109, KYSE150 and KYSE180, were infected with AdGFP and AdBMP9 separately, using those uninfected as blank control. The transcription levels of BMP9 mRNA in various groups were determined by RT-PCR method, while the proliferative activities of cells 0 - 96 h after infection were determined by MTT method, while the colony formation abilities by plate colony formation assay, the migration abilities by wound healing assay, and the invasion abilities by transwell migration assay. The ECA109 ceils in three groups were determined for cell cycle. Results The transcription levels of BMP9 mRNA in esophageal cancer cell lines infected with AdBMP9 increased significantlg. After over-expression of BMP9, all the proliferative activities of the three cell strains decreased significantly as compared with those infected with AdGFP and those uninfected （P 〈 0. 05）, while the colony formation ability decreased, with a decrease rate of more than 70%, the cell aggregates were small in size, and the migration and invasion abilities decreased. However, the percentage of ECAI09 cells at Gl phase increased significantly, while that at S phase decreased significantly （P 〈 0. 05）. Conclusion BMP9 significantly inhibited the proliferation, cloning, migration and invasion of esophageal cancer cells, and arrested the cells at G1 phase.

关 键 词：骨形态发生蛋白9 食管鳞状细胞癌 抑制作用 

分 类 号：R735.1[医药卫生—肿瘤]