热习服兔实验模型的建立及其病理生理特征  

Establishment of heat acclimatization model in rabbits and its pathophysilogical characteristics

作　　者：王涛[1] 王静[1] 王尚[1] 李佩尧[1] 张文成[1] 赵小玲[1] 汪海[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国应用生理学杂志》2013年第5期473-476,共4页Chinese Journal of Applied Physiology

摘　　要：目的:建立有效稳定的兔热习服模型,为热习服机制研究提供实验基础。方法:16只兔随机分为热习服组和对照组(n=8)。热习服组置于干球温度(36±1)℃,湿球温度(29±0.5)℃,黑球温度(40±1.0)℃的动物高温模拟舱,100 min/d,持续20 d;对照组置于温度20℃左右,相对湿度<60%的室内,持续20 d,第21天置于动物高温模拟舱与热习服组共同进行热暴露,条件同热习服组,实时监测肛温。检测对照组第1天及热习服组热暴露前、第1、11和21天热暴露后静脉血中肿瘤坏死因子α(TNF-α)、热休克蛋白70(HSP70)及白介素6(IL-6)表达水平。结果:①肛温:对照组20 d肛温无明显变化。热习服组,第1天热暴露后,肛温升高(2.07±0.43)℃,显著高于对照组;第11天暴露后肛温升高(1.78±0.37)℃,较第1天升高幅度降低(0.29±0.09)℃;第21天暴露后肛温升高(1.52±0.29)℃,较第1天升高幅度降低(0.55±0.14)℃(P<0.05);第21天对照组与热习服组同时热暴露,热习服组较对照组肛温升高幅度降低(0.53±0.14)℃(P<0.05)。②血清学评价:兔血清TNF-α第1天暴露后显著高于对照组与热暴露前(P<0.05),第11、21天热暴露后降回热暴露前水平。血清IL-6第1天热暴露后兔显著高于对照组与热暴露前(P<0.05),第11、21天热暴露后亦高于热暴露前(P<0.05),但升高幅度明显减缓。血清HSP70水平第1天热暴露后开始升高,第11、21天热暴露后显著高于对照组与热暴露前(P<0.05)。结论:通过连续规律的热暴露,动物肛温升高幅度降低,细胞反应趋于稳定,表明在热环境中连续反复地短期热暴露,可以提高机体的热耐受能力从而达到热习服。Objective： To establish an effective and stable rabbit heat acclimatization model for the experiment of heat acclimatization mechanisms. Methods： Sixteen healthy male rabbits were divided into heat acclimatization group and control group randomly （ n = 8）. Heat acclimatization（HA） group was kept in simulation chamber with dry bulb temperature of （36 ± 1）℃, wet bulb temperature of （29 ± 0.5）℃,black-bulb temperature of（40：1： 1.O）℃, 100 rain/day for 21 days. Control group was kept in the room with temperature of 20℃ and relative humidity 〈 60% during 20 days, then removed into simulation chamber on day 21 to estimate and monitor the rectal temperature together with the heat acclimatization group. Venous blood of control and heat acclimatization group before and after heat exposure on the 1st day, 11th day and 21st day were collected to detect levels of tumor necrosis factor-α （TNF-α）, interleukin 6（IL-6） and heat shock protein 70 （HSP70） by ELISA analysis. Results： ①Rectal temperature： There was no significant change in control group during 21 days. In heat acclimatization group, it increased （2.07 ±0.43）℃ after the 1st exposure, and increased （1.78 ± 0.37）℃ after the 11th exposure, the range of increasing decreased （0.29 ± 0.09） ℃. After the 21st exposure, it increased （ 1.52 ± 0.29） ℃, which was （0.55 ± 0.14） ℃ lower than that of the 1st （ P 〈 0.05） ,and （0.53 ± 0.14） ℃ lower to that of the control group under 1st heat stress （ P 〈 0.05） ; ②The level of TNF-α after the 1st exposure increased significantly （ P 〈 0.05）, but didn＇ t raise along with the exposure times. And fell back to the original level after the 11th and 21st exposure. Compared with control group, the level of IL-6 increased after the 1st, 11th and 21st exposure （ P 〈 0.05）, and maintained highly after the 11th and 21st exposure. Compared with the control group, the level of HSP70 increased dramatically with the heat ex

关 键 词：热习服 动物模型 肛温 热休克蛋白 

分 类 号：R-332[医药卫生]